TNFAIP1 Mediates Formaldehyde-Induced Neurotoxicity by Inhibiting the Akt/CREB Pathway in N2a Cells
| العنوان: | TNFAIP1 Mediates Formaldehyde-Induced Neurotoxicity by Inhibiting the Akt/CREB Pathway in N2a Cells |
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| المؤلفون: | Chenxi Wei, Ning Liu, Feng Qiu, Yubo Zhou, Junzhi Yi, Min Zhu, Shuanglin Xiang, Pan Shu |
| المصدر: | Neurotoxicity Research. 38:184-198 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Small interfering RNA, Neurite, Cell Survival, Apoptosis, Toxicology, CREB, Mice, 03 medical and health sciences, 0302 clinical medicine, Cell Line, Tumor, Formaldehyde, Neurites, medicine, Animals, Viability assay, Cyclic AMP Response Element-Binding Protein, Protein kinase B, Adaptor Proteins, Signal Transducing, biology, Chemistry, Brain-Derived Neurotrophic Factor, General Neuroscience, Neurotoxicity, medicine.disease, Cell biology, 030104 developmental biology, biology.protein, Neurotoxicity Syndromes, Signal transduction, Proto-Oncogene Proteins c-akt, 030217 neurology & neurosurgery, Signal Transduction |
| الوصف: | Formaldehyde (FA) is a common air pollutant. Exposure to exogenous FA can cause damage to the nervous system, such as learning and memory impairment, balance dysfunction, and sleep disorders. Excessive production of endogenous FA also causes memory impairment and is thought to be associated with Alzheimer's disease (AD). Tumor necrosis factor alpha-induced protein 1 (TNFAIP1) plays a crucial role in neurodevelopment and neurological diseases. However, the role of TNFAIP1 in FA-induced neurotoxicity is unclear. Herein, using a mouse neuroblastoma cell line (N2a cells), we explored the mechanism of TNFAIP1 in FA-induced neurotoxicity, the involvement of the Akt/CREB signaling pathway, and how the expression of TNFAIP1 is regulated by FA. We found that exposure to 100 μM or 200 μM FA for 24 h led to decreased cell viability, increased cell apoptosis and neurite retraction, increased reactive oxygen species (ROS) levels, upregulated protein expression of TNFAIP1 and decreased the levels of phosphorylated Akt and CREB in the Akt/CREB pathway. Knockdown of TNFAIP1 using a TNFAIP1 small interfering RNA (siRNA) expression vector prevented FA from inhibiting the Akt/CREB pathway, thus reducing cell apoptosis and restoring cell viability and neurite outgrowth. Clearance of ROS by vitamin E (Vit E) repressed the FA-mediated upregulation of TNFAIP1 expression. These results suggest that FA increases the expression of TNFAIP1 by inducing oxidative stress and that upregulated TNFAIP1 then inhibits the Akt/CREB pathway, consequently leading to cell apoptosis and neurite retraction. Therefore, TNFAIP1 is a potential target for alleviating FA-induced neurotoxicity and related neurological disorders. |
| تدمد: | 1476-3524 1029-8428 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e78ca7dbf1cc8b7eab1a98e2d4162884 https://doi.org/10.1007/s12640-020-00199-9 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....e78ca7dbf1cc8b7eab1a98e2d4162884 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14763524 10298428 |
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