Rapid Screening Alpha-Glucosidase Inhibitors from Polygoni Vivipari Rhizoma by Multi-Step Matrix Solid-Phase Dispersion, Ultrafiltration and HPLC
| العنوان: | Rapid Screening Alpha-Glucosidase Inhibitors from Polygoni Vivipari Rhizoma by Multi-Step Matrix Solid-Phase Dispersion, Ultrafiltration and HPLC |
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| المؤلفون: | Zhengming Qian, Haoxiang Li, Weifeng Fan, Zhuobin He, Yuansheng Zou, Qianhui Shen, Quanxi Mei, Guoying Tan |
| المصدر: | Molecules Volume 26 Issue 20 Molecules, Vol 26, Iss 6111, p 6111 (2021) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Ultrafiltration, Pharmaceutical Science, Hyperoside, High-performance liquid chromatography, Article, Analytical Chemistry, Matrix (chemical analysis), Polygoni Vivipari Rhizoma, Rutin, chemistry.chemical_compound, QD241-441, MSPD, Drug Discovery, Diabetes Mellitus, Humans, Physical and Theoretical Chemistry, alpha-glucosidase inhibitors, Chromatography, High Pressure Liquid, Chromatography, Plant Extracts, Organic Chemistry, Extraction (chemistry), alpha-Glucosidases, Polygonaceae, Molecular Docking Simulation, chemistry, Chemistry (miscellaneous), ultrafiltration, Molecular Medicine, HPLC, Quercetin, Kaempferol, Rhizome, Drugs, Chinese Herbal |
| الوصف: | Polygoni Vivipari Rhizoma (PVR), the dried root of Polygonum viviparum, has been used as herbal medicine in China for a long time. In the present study, a new method based on multi-step matrix solid-phase dispersion (MSPD), ultrafiltration and high performance liquid chromatography (HPLC) for screening alpha-glucosidase inhibitors (AGIs) from PVR was proposed. First, three different PVR extractions were prepared by multi-step MSPD with 15% methanol, 60% methanol and 100% methanol. Second, the alpha-glucosidase inhibition tests for the three extracts were carried out, and the 60% methanol extraction showed the best activity. Then, the AGIs screening experiment was performed with ultrafiltration and HPLC analysis using the 60% methanol extraction. Seven binding components (quercetin−3−O−vicianoside, quercetin 3−O−neohesperidoside, rutin, hyperoside, quercetin 3−O−glucuronide, luteolin−7−O−neohesperidoside, kaempferol 3−glucuronide) were found. These seven components were further validated as the AGIs by molecular docking analysis. The developed method was a rapid and efficient tool for screening AGIs from PVR, which provided scientific data for the bioactive components study of PVR. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1420-3049 |
| DOI: | 10.3390/molecules26206111 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7daabf6102aba2188582f92dd43170f |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e7daabf6102aba2188582f92dd43170f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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| DOI: | 10.3390/molecules26206111 |