Prevalence of mupirocin resistance among invasive coagulase-negative staphylococci and methicillin-resistant Staphylococcus aureus (MRSA) in France: emergence of a mupirocin-resistant MRSA clone harbouring mupA
| العنوان: | Prevalence of mupirocin resistance among invasive coagulase-negative staphylococci and methicillin-resistant Staphylococcus aureus (MRSA) in France: emergence of a mupirocin-resistant MRSA clone harbouring mupA |
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| المؤلفون: | Richard Bonnet, Hélène Jean-Pierre, Anne-Sophie Bourrel, J. Etienne, Y. Rio, Jacques Tankovic, M. Biendo, Elisabeth Chachaty, Jean-Winoc Decousser, Véronique Vernet-Garnier, Julien Potier, Nejla Aissa, Alain Lozniewski, Alain Fiacre, Marie-Hélène Nicolas-Chanoine, Frédéric Laurent, Patrice Nordmann, F. Girard-Pipau, Jean-Philippe Lavigne, C. Plouzeau-Jayle, Christian Cattoen, François Vandenesch, Vincent Cattoir, Florence Doucet-Populaire, C. Eloy, Jocelyne Caillon, François Caron, T. Roussel-Gaillard, François Jehl, J. Texier-Maugein, Max Maurin, Laurent Bret, Grégoire Aubert, M. Archambaud, Marie-Cécile Ploy, C. Loiez-Durocher, Isabelle Patry, Gerard Lina, H. Courtade, C. Buisson-Touati, H. Rostane, G. Blanchard-Marche, Nicolas Fortineau, Roland Leclercq, Nadège Bourgeois-Nicolaos, Philippe Morand, Marine Desroches, Frédéric Robin |
| المساهمون: | Hôpital Antoine Béclère, Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Immunité infection vaccination (I2V), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM), Bactéries, Pathogènes et Santé (UBaPS), Faculté de Pharmacie, Université Paris-Sud - Paris 11 (UP11)-Université Paris-Sud - Paris 11 (UP11), CHU Henri Mondor, Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques (RESINFIT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-CHU Limoges, Service de Bactériologie, Virologie, Hygiène [CHU Limoges], CHU Limoges, Novartis, France |
| المصدر: | Journal of Antimicrobial Chemotherapy Journal of Antimicrobial Chemotherapy, Oxford University Press (OUP), 2013, 68 (8), pp.1714-1717. ⟨10.1093/jac/dkt085⟩ |
| بيانات النشر: | Oxford University Press (OUP), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | DNA, Bacterial, Microbiology (medical), Staphylococcus, Mupirocin, Microbial Sensitivity Tests, Biology, Staphylococcal infections, medicine.disease_cause, Polymerase Chain Reaction, Microbiology, law.invention, 03 medical and health sciences, chemistry.chemical_compound, Bacterial Proteins, law, Drug Resistance, Bacterial, medicine, Pharmacology (medical), Prospective Studies, Polymerase chain reaction, 030304 developmental biology, Lyon clone, Pharmacology, Molecular Epidemiology, 0303 health sciences, 030306 microbiology, Broth microdilution, Nuclear Proteins, invasive staphylococci, Staphylococcal Infections, Microarray Analysis, medicine.disease, Methicillin-resistant Staphylococcus aureus, Anti-Bacterial Agents, Electrophoresis, Gel, Pulsed-Field, 3. Good health, Molecular Typing, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Infectious Diseases, chemistry, Staphylococcus aureus, [SDV.IMM]Life Sciences [q-bio]/Immunology, decolonization, lipids (amino acids, peptides, and proteins), France, Coagulase |
| الوصف: | OBJECTIVES Mupirocin is the cornerstone of decolonization regimens, a successful strategy to prevent healthcare-associated staphylococcal infections. Several recent studies have reported alarming results: (i) an extending reservoir of mupA, the ancestral mobile resistance gene, among coagulase-negative staphylococci (CoNS); (ii) the emergence of a new resistance gene (mupB); and (iii) a growing number of mupirocin-resistant methicillin-resistant Staphylococcus aureus (MRSA), including highly pathogenic clones. We performed a nationwide prospective study in France to detect such trends among invasive staphylococci. METHODS Between October 2011 and February 2012, 367 MRSA and 708 CoNS invasive isolates were collected from 37 hospitals and analysed centrally. Mupirocin MICs were determined using the broth microdilution method. mupA/B PCR was performed for resistant isolates (MIC >1 mg/L). Genetic relatedness between mupirocin-resistant MRSA isolates was determined by PFGE analysis and related isolates were tested by microarray. RESULTS Among MRSA isolates 2.2% (n = 8) were classified as mupirocin resistant; 1.4% (n = 5) showing low-level resistance (MIC ≤256 mg/L) and 0.8% (n = 3) high-level resistance (MIC >256 mg/L). Only the latter isolates carried mupA. A clonal relationship was identified between two mupA-negative MRSA from the same hospital and three mupA-positive MRSA from three distant towns; these three isolates belonged to the Lyon clone. Mupirocin resistance was identified in 10.3% of CoNS, mainly highly resistant mupA-positive isolates (5.6%). The mupB gene was not detected in mupirocin-resistant MRSA or CoNS. CONCLUSIONS This first large national study indicates the need for thorough epidemiological monitoring and a stewardship programme to prevent and detect mupirocin resistance in staphylococci. |
| تدمد: | 1460-2091 0305-7453 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e804f7fea2d9ea9016423d1af55564aa https://doi.org/10.1093/jac/dkt085 |
| رقم الأكسشن: | edsair.doi.dedup.....e804f7fea2d9ea9016423d1af55564aa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602091 03057453 |
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