Quantification of wild-type and radiation attenuated Plasmodium falciparum sporozoite motility in human skin
| العنوان: | Quantification of wild-type and radiation attenuated Plasmodium falciparum sporozoite motility in human skin |
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| المؤلفون: | Fijs W. B. van Leeuwen, Mark Meijhuis, Martijn W. Vos, Severine Chevalley-Maurel, Munisha S. Ganesh, Blandine Franke-Fayard, Clarize M. de Korne, Diego Staphorst, Els Baalbergen, Matthias N. van Oosterom, Beatrice M. F. Winkel, Koen J. Dechering, Meta Roestenberg |
| المصدر: | Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) Scientific Reports Scientific Reports, 9. NATURE PUBLISHING GROUP |
| بيانات النشر: | Nature Publishing Group, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, 030231 tropical medicine, Green Fluorescent Proteins, Plasmodium falciparum, Motility, lcsh:Medicine, Human skin, Biology, Article, Host-Parasite Interactions, 03 medical and health sciences, 0302 clinical medicine, Anopheles, medicine, Image Processing, Computer-Assisted, Parasite hosting, Animals, Humans, lcsh:Science, Skin, Infectivity, Multidisciplinary, Organisms, Genetically Modified, Malaria vaccine, lcsh:R, Wild type, biology.organism_classification, medicine.disease, Cell biology, 030104 developmental biology, Sporozoites, Preclinical research, Infectious diseases, Parasitology, lcsh:Q, Malaria, Software |
| الوصف: | Given the number of global malaria cases and deaths, the need for a vaccine against Plasmodium falciparum (Pf) remains pressing. Administration of live, radiation-attenuated Pf sporozoites can fully protect malaria-naïve individuals. Despite the fact that motility of these attenuated parasites is key to their infectivity and ultimately protective efficacy, sporozoite motility in human tissue (e.g. skin) remains wholly uncharacterized to date. We show that the ability to quantitatively address the complexity of sporozoite motility in human tissue provides an additional tool in the development of attenuated sporozoite vaccines. We imaged Pf movement in the skin of its natural host and compared wild-type and radiation-attenuated GFP-expressing Pf sporozoites. Using custom image analysis software and human skin explants we were able to quantitatively study their key motility features. This head-to-head comparison revealed that radiation attenuation impaired the capacity of sporozoites to vary their movement angle, velocity and direction, promoting less refined movement patterns. Understanding and overcoming these changes in motility will contribute to the development of an efficacious attenuated parasite malaria vaccine. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e836275da42f2928c70731e17c73bf5e http://link.springer.com/article/10.1038/s41598-019-49895-3 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e836275da42f2928c70731e17c73bf5e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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