Steering protein and salt ad- and desorption by an electrical switch applied to polymer-coated electrodes
| العنوان: | Steering protein and salt ad- and desorption by an electrical switch applied to polymer-coated electrodes |
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| المؤلفون: | Pina A. Fritz, Mary B. Chan-Park, Louis C. P. M. de Smet, Penghui Zhang, C.G.P.H. Schroën, Sevil Sahin, Remko M. Boom, Tom Bruschinski |
| المصدر: | Separation and Purification Technology 250 (2020) Separation and Purification Technology, 250 |
| بيانات النشر: | Elsevier BV, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Filtration and Separation, Protein desorption, 02 engineering and technology, Electrochemistry, Analytical Chemistry, Whey protein isolate, Polystyrene sulfonate, Capacitive deionization, chemistry.chemical_compound, Electrochemical protein separation, Adsorption, 020401 chemical engineering, Desorption, Protein purification, medicine, 0204 chemical engineering, Food Process Engineering, VLAG, biology, Organic Chemistry, Polyelectrolyte, 021001 nanoscience & nanotechnology, Organische Chemie, chemistry, Chemical engineering, Carbon electrodes, Electrode, biology.protein, 0210 nano-technology, Activated carbon, medicine.drug |
| الوصف: | Although solid-phase chromatography is a well-established method for protein separation, chemically intensive and often costly regeneration steps are needed to make reuse of the adsorbent possible. Here, we demonstrate the use of electrochemical principles as sustainable alternative. We make use of spontaneous adsorption of proteins to solid electrodes and reverse this process by applying an electric potential to regenerate the interface. This allows for adsorption of proteins to take place at 0 V difference between the electrodes, due to electrostatic interactions between the protein and the electrode surface. The desorption is then triggered by applying a potential difference (−1.2 V) between the electrodes. It is demonstrated that the incorporation of negatively charged polystyrene sulfonate (PSS) or positively charged polydiallyldimethylammonium chloride (PDMAC) in or on top of the respective activated carbon electrodes increases the amount of exchanged protein from 1 to 10 mg g−1, as compared to simple activated carbon electrodes. Interestingly, salt ad- and desorption occurs in opposite cycles compared to protein ad- and desorption, resulting in simultaneous concentration and desalting of the protein when 0 V is applied. On top of that, we also found that an enrichment in β-lactoglobulin could be achieved starting from whey protein isolate. These results clearly demonstrate that electrochemical technologies can be used not only for protein separation (including removal of salt), but also for protein fractionation, while not requiring solvent use. |
| وصف الملف: | application/pdf |
| تدمد: | 1383-5866 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8742ba28c8e0d69ddfb5927bd0002d9 https://doi.org/10.1016/j.seppur.2020.117195 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e8742ba28c8e0d69ddfb5927bd0002d9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13835866 |
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