Integrated Analysis of the Metabolome and Transcriptome on Anthocyanin Biosynthesis in Four Developmental Stages of Cerasus humilis Peel Coloration
| العنوان: | Integrated Analysis of the Metabolome and Transcriptome on Anthocyanin Biosynthesis in Four Developmental Stages of Cerasus humilis Peel Coloration |
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| المؤلفون: | Yixin Zhang, Xingshun Song, Di Wang, Shaoyu Lang, Xiaolong Ji, Jing Ren |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 11880, p 11880 (2021) International Journal of Molecular Sciences Volume 22 Issue 21 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | QH301-705.5, Cyanidin, Biology, Article, Catalysis, Pelargonidin, anthocyanin, Anthocyanins, Inorganic Chemistry, Transcriptome, chemistry.chemical_compound, Metabolomics, Cerasus humilis, Gene Expression Regulation, Plant, Botany, Metabolome, MYB, pigmentation, Cultivar, Physical and Theoretical Chemistry, Biology (General), Molecular Biology, QD1-999, Spectroscopy, metabolites, Plant Proteins, Organic Chemistry, fungi, Gene Expression Regulation, Developmental, food and beverages, General Medicine, Computer Science Applications, Chemistry, chemistry, Fruit, Anthocyanin, Prunus, transcriptome |
| الوصف: | Cerasus humilis is a unique dwarf shrub and fruit color is an important trait in the species. In this study, we evaluated the transcriptomic and metabolomic profiles of the plant at different developmental stages to elucidate the mechanism underlying color formation. In a metabolomics analysis, 16 anthocyanin components were identified at four developmental stages, and high levels of cyanidin O-syringic acid and pelargonidin 3-O-beta-d-glucoside (callitephin chloride) were correlated with the reddening of the fruit peel. A co-expression analysis revealed that ANS and UFGT play key roles in pigmentation (PCC > 0.82). Additionally, transcriptome data showed that most anthocyanin biosynthetic genes and two MYB transcription factors were significantly up-regulated. QRT-PCR results for these differentially expressed genes were generally consistent with the high-throughput sequencing. Moreover, the overexpression of ChMYB1 (TRINITY_DN21536_c0_g1) in apple calli could contribute to the accumulation of anthocyanin. It was also found that UFGT (TRINITY_DN19893_c1_g5) and ChMYB1 (TRINITY_DN21536_c0_g1) have similar expression patterns. These findings provide insight into the mechanisms underlying anthocyanin accumulation and coloration during fruit peel development, providing a basis for the breeding of anthocyanin-rich C. humilis cultivars. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1661-6596 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e89ef106eb835469e80956284a3f8978 https://www.mdpi.com/1422-0067/22/21/11880 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e89ef106eb835469e80956284a3f8978 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 14220067 |
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