YUCCA-Mediated Biosynthesis of the Auxin IAA Is Required during the Somatic Embryogenic Induction Process in Coffea canephora
| العنوان: | YUCCA-Mediated Biosynthesis of the Auxin IAA Is Required during the Somatic Embryogenic Induction Process in Coffea canephora |
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| المؤلفون: | Ligia Brito-Argáez, Víctor M. Loyola-Vargas, Cleyre Pérez-Hernández, Miguel A. Uc-Chuc, Rosa M. Galaz-Ávalos, Victor Aguilar-Hernández |
| المصدر: | International Journal of Molecular Sciences Volume 21 Issue 13 International Journal of Molecular Sciences, Vol 21, Iss 4751, p 4751 (2020) |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Somatic embryogenesis, Coffea, 01 natural sciences, localization, Mixed Function Oxygenases, lcsh:Chemistry, chemistry.chemical_compound, Plant Growth Regulators, Arabidopsis, Gene expression, heterocyclic compounds, yucasin, Enzyme Inhibitors, lcsh:QH301-705.5, Spectroscopy, Plant Proteins, chemistry.chemical_classification, biology, YUCCA, food and beverages, General Medicine, Computer Science Applications, Biochemistry, Multigene Family, Plant Somatic Embryogenesis Techniques, Genes, Plant, Catalysis, Article, Inorganic Chemistry, 03 medical and health sciences, Coffea canephora, Biosynthesis, Auxin, Physical and Theoretical Chemistry, Molecular Biology, Indoleacetic Acids, Gene Expression Profiling, Organic Chemistry, Tryptophan, Monooxygenase, somatic embryogenesis, Triazoles, biology.organism_classification, Biosynthetic Pathways, 030104 developmental biology, chemistry, lcsh:Biology (General), lcsh:QD1-999, Kinetin, auxin, 010606 plant biology & botany |
| الوصف: | Despite the existence of considerable research on somatic embryogenesis (SE), the molecular mechanism that regulates the biosynthesis of auxins during the SE induction process remains unknown. Indole-3-acetic acid (IAA) is an auxin that is synthesized in plants through five pathways. The biosynthetic pathway most frequently used in this synthesis is the conversion of tryptophan to indol-3-pyruvic acid (IPA) by tryptophan aminotransferase of Arabidopsis (TAA) followed by the conversion of IPA to IAA by enzymes encoded by YUCCA (YUC) genes of the flavin monooxygenase family however, it is unclear whether YUC-mediated IAA biosynthesis is involved in SE induction. In this study, we report that the increase of IAA observed during SE pre-treatment (plants in MS medium supplemented with 1-naphthaleneacetic acid (NAA) 0.54 µ M and kinetin (Kin) 2.32 µ M for 14 days) was due to its de novo biosynthesis. By qRT-PCR, we demonstrated that YUC gene expression was consistent with the free IAA signal found in the explants during the induction of SE. In addition, the use of yucasin to inhibit the activity of YUC enzymes reduced the signal of free IAA in the leaf explants and dramatically decreased the induction of SE. The exogenous addition of IAA restored the SE process in explants treated with yucasin. Our findings suggest that the biosynthesis and localization of IAA play an essential role during the induction process of SE in Coffea canephora. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9b24eddcfdd15b09dc6e52540a2da1a http://europepmc.org/articles/PMC7369726 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....e9b24eddcfdd15b09dc6e52540a2da1a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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