E6-associated transcription patterns in human papilloma virus 16-positive cervical tissues

التفاصيل البيبلوغرافية
العنوان: E6-associated transcription patterns in human papilloma virus 16-positive cervical tissues
المؤلفون: Jun Chen, Ruanmin Zou, Lifang Zhang, Xiangyang Xue, Kezhi Lin, Xulian Lu
المصدر: Oncology Letters
بيانات النشر: Spandidos Publications, 2014.
سنة النشر: 2014
مصطلحات موضوعية: Cervical cancer, Cancer Research, Pathology, medicine.medical_specialty, Oncogene, cervical cancer, amplification of papillomavirus oncogene transcripts, Articles, Cell cycle, Biology, medicine.disease, papillomavirus, Molecular biology, HPV-16, Oncology, Transcription (biology), Agarose gel electrophoresis, RNA splicing, Gene expression, medicine, Gene
الوصف: The change in transcription pattern induced by post-transcriptional RNA splicing is an important mechanism in the regulation of the early gene expression of human papilloma virus (HPV). The present study was conducted to establish a method to specifically amplify HPV-16 E6-associated transcripts. The E6-related transcripts from 63 HPV-16-positive cervical tumor tissue samples were amplified, consisting of eight cases of low-risk intraepithelial lesions, 38 cases of high-risk intraepithelial lesions and 17 cases of cervical cancer (CxCa). The appropriate amplified segments were recovered following agarose gel electrophoresis, and subjected to further sequencing and sequence alignment analysis. Six groups of E6 transcription patterns were identified from HPV-16-positive cervical tumor tissue, including five newly-discovered transcripts. Different HPV-16 E6-associated transcription patterns were detected during the development of CxCa. Over the course of the progression of the low-grade squamous intraepithelial lesions to CxCa, the specific HPV-16 E6-associated transcription patterns and the dominant transcripts were all different. As indicated by this study, the transcription pattern of the E6 early gene of HPV-16 was closely associated with the stages of cervical carcinogenesis, and may also be involved in the development of CxCa.
تدمد: 1792-1082
1792-1074
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea09971a9a41556a6a0660beaf01c790
https://doi.org/10.3892/ol.2014.2698
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....ea09971a9a41556a6a0660beaf01c790
قاعدة البيانات: OpenAIRE