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IBD: Role of intestinal compartments in the mucosal immune response

التفاصيل البيبلوغرافية
العنوان: IBD: Role of intestinal compartments in the mucosal immune response
المؤلفون: Gaetano Iaquinto, Giuseppe Mazzarella, Vera Rotondi Aufiero, Giuseppe Iacomino, Raffaele Melina, Fabio Silvio Taccone, Nicola Giardullo, Nunzia Iannaccone, Giovanni De Chiara, Antonella Venezia
المصدر: Immunobiology (1979) (2020). doi:10.1016/j.imbio.2019.09.008
info:cnr-pdr/source/autori:Iacomino G.; Rotondi Aufiero V.; Iannaccone N.; Melina R.; Giardullo N.; De Chiara G.; Venezia A.; Taccone F.S.; Iaquinto G.; Mazzarella G./titolo:IBD: Role of intestinal compartments in the mucosal immune response/doi:10.1016%2Fj.imbio.2019.09.008/rivista:Immunobiology (1979)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume
بيانات النشر: Elsevier BV, 2020.
سنة النشر: 2020
مصطلحات موضوعية: Adult, Male, 0301 basic medicine, XBP1, IBD, Immunology, Laser Capture Microdissection, Biology, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Immune system, Downregulation and upregulation, medicine, Humans, Immunology and Allergy, TH17, Intestinal Mucosa, Aged, Laser capture microdissection, Lamina propria, FOXP3, LCM, Hematology, Middle Aged, Th1 Cells, Inflammatory Bowel Diseases, Immunohistochemistry, Epithelium, TH1, Treg, 030104 developmental biology, medicine.anatomical_structure, Unfolded protein response, Cytokines, Th17 Cells, Female, ER stress, Leukocyte Elastase, 030215 immunology
الوصف: Background and aims Laser capture microdissection (LCM) is a powerful tool for the isolation of specific tissue compartments. We aimed to investigate the mucosal immune response that takes place in different intestinal compartments of IBD patients, dissected by LCM, analyzing cytokines expression profile and endoplasmic reticulum (ER) stress markers. Methods Frozen sections of gut were obtained from patients with Crohn’s disease (CD), ulcerative colitis (UC) and from controls. Using LCM, surface epithelium (SE) and lamina propria (LP) compartments were isolated and total RNA extracted. The relative expression of Th1, Th17 and Treg cytokines was evaluated by quantitative reverse transcriptase real-time PCR (qRT-PCR), in addition to the assessment of mRNA splicing of the transcription factor X-box binding protein-1 (XBP1). Human neutrophil elastase (HNE) and the transcription factor forkhead box P3 (Foxp3) were also analyzed by immunohistochemistry. Results The increased expression of IL-17 was observed in both intestinal compartments of IBD patients when compared to controls. IFN- γ, TNF-α , IL-10, HNE and Foxp3 were overexpressed in the LP compartment of both IBD patients as compared to controls. An upregulation of IFN-γ and an infiltration of HNE+ cells was found in the SE of patients with UC. Splicing of XBP1 mRNA was recognized in both intestinal compartments of IBD patients when compared to controls. Conclusions In IBD patients, both intestinal compartments are involved in Th17 response, whereas, LP compartment plays a prominent role in Th1 and Treg immune responses. Nevertheless, high level of IFN- γ was found in the SE of UC patients, suggesting that this compartment is involved in the Th1 immune response. Our data also suggested that ER stress signalling is active in both LP and SE compartment of IBD patients, thus advocating that ER stress and immunity are intertwined.
تدمد: 0171-2985
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea9850d2eb03c0242c4fb35a3a087f19
https://doi.org/10.1016/j.imbio.2019.09.008
حقوق: RESTRICTED
رقم الأكسشن: edsair.doi.dedup.....ea9850d2eb03c0242c4fb35a3a087f19
قاعدة البيانات: OpenAIRE
الوصف
تدمد:01712985