Extracellular mRNA transported to the nucleus exerts translation-independent function
| العنوان: | Extracellular mRNA transported to the nucleus exerts translation-independent function |
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| المؤلفون: | Masayoshi Kato, Noriki Iwai, Sachie Hiratsuka, Taishi Mishima, Hideki Sanjo, Satoshi Takahashi, Ken ichi Ito, Yoshiro Maru, Toshimitsu Matsui, Osamu Takeuchi, Toshifumi Takao, Yuta Matsunaga, Takeshi Tomita, Hiroyuki Oikawa, Takashi Mino, Kentaro Minagawa |
| المصدر: | Nature Communications, Vol 12, Iss 1, Pp 1-19 (2021) Nature Communications |
| بيانات النشر: | Nature Portfolio, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Cancer microenvironment, 0301 basic medicine, Untranslated region, Science, Interleukin-1beta, General Physics and Astronomy, Antineoplastic Agents, Cell Cycle Proteins, Article, General Biochemistry, Genetics and Molecular Biology, Cell membrane, Mice, 03 medical and health sciences, 0302 clinical medicine, Cell Line, Tumor, Endoribonucleases, Extracellular, medicine, Animals, Humans, RNA, Messenger, 3' Untranslated Regions, Cell Nucleus, Multidisciplinary, Chemistry, Three prime untranslated region, Cell Membrane, RNA-Binding Proteins, General Chemistry, Cell biology, Killer Cells, Natural, Cell nucleus, 030104 developmental biology, medicine.anatomical_structure, Cell culture, Culture Media, Conditioned, 030220 oncology & carcinogenesis, Cancer cell, Extracellular signalling molecules, Extracellular Space, Nucleus, Protein Binding |
| الوصف: | RNA in extracellular vesicles (EVs) are uptaken by cells, where they regulate fundamental cellular functions. EV-derived mRNA in recipient cells can be translated. However, it is still elusive whether “naked nonvesicular extracellular mRNA” (nex-mRNA) that are not packed in EVs can be uptaken by cells and, if so, whether they have any functions in recipient cells. Here, we show the entrance of nex-mRNA in the nucleus, where they exert a translation-independent function. Human nex-interleukin-1β (IL1β)-mRNA outside cells proved to be captured by RNA-binding zinc finger CCCH domain containing protein 12D (ZC3H12D)-expressing human natural killer (NK) cells. ZC3H12D recruited to the cell membrane binds to the 3′-untranslated region of nex-IL1β-mRNA and transports it to the nucleus. The nex-IL1β-mRNA in the NK cell nucleus upregulates antiapoptotic gene expression, migration activity, and interferon-γ production, leading to the killing of cancer cells and antimetastasis in mice. These results implicate the diverse actions of mRNA. Nonvesicular extracellular RNA (nex-RNA) that are not packed in extracellular vesicles is detected outside the cell, but it is poorly understood. Here the authors report that nex-RNA is captured by a zinc finger protein and transported to the nucleus to enhance antimetastatic characters of the cell. |
| اللغة: | English |
| تدمد: | 2041-1723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eacaa04621d042b9f6d8c26cc3248843 https://doaj.org/article/a75fe0cb0a7242eda55465abbb5ce4d8 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....eacaa04621d042b9f6d8c26cc3248843 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 20411723 |
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