Decelluarization and Reseeding of Porcine Colon with Human CaCo-2 Cells for Whole-Scale Organ Engineering Applications
| العنوان: | Decelluarization and Reseeding of Porcine Colon with Human CaCo-2 Cells for Whole-Scale Organ Engineering Applications |
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| المؤلفون: | Yumi Suh, Rahul Nayak, Julie Haduong, Vinay Viswanadham |
| المصدر: | Haduong, Julie; Nayak, Rahul; Suh, Yumi; & Viswanadham, Vinay. (2014). Decelluarization and Reseeding of Porcine Colon with Human CaCo-2 Cells for Whole-Scale Organ Engineering Applications. Berkeley Scientific Journal, 19(1). Retrieved from: http://www.escholarship.org/uc/item/3b0743s3 |
| بيانات النشر: | California Digital Library (CDL), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Organ engineering, Scaffold, Decellularization, Caco-2, Chemistry, Regeneration (biology), Immunohistochemistry, Forestry, Plant Science, Decellularized scaffold, Stem cell, Agronomy and Crop Science, Biomedical engineering |
| الوصف: | A new approach to whole-scale organ engineering is the seeding of stem cells upon a decellularized scaffold of a desired organ. Current efforts seek to use this method for whole-scale organ engineering. Here, we report a novel decellularizationrecellularization approach to engineering a human colonic graft with human enterocytes and xenogeneic scaffold. Porcine colon tissue was decellularized with protein-solubilizing agents and recellularized by incubation with Caco-2. Immunohistochemistry, DNA staining, and SEM were used to study the effects upon the scaffold. Exposure to 2%SDS-0.5%Triton solution for four hours resulted in the best decellularization and preservation of microstructure, while tissues treated with 2%SDS-0.5%Triton and 0.05%Trypsin/EDTA supported the best recellularization. Our method provides a potential strategy for streamlined regeneration of a viable colonic lumen from xenogeneic and allogeneic sources while maintaining colon microstructure and potential for recellularization. |
| وصف الملف: | application/pdf |
| تدمد: | 2373-8146 1097-0967 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eaec33604aa588bc33ef391c14dd5d0c https://doi.org/10.5070/bs3191025164 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....eaec33604aa588bc33ef391c14dd5d0c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23738146 10970967 |
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