Nanodiamonds-induced effects on neuronal firing of mouse hippocampal microcircuits
| العنوان: | Nanodiamonds-induced effects on neuronal firing of mouse hippocampal microcircuits |
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| المؤلفون: | S. Ditalia Tchernij, Chiara Calorio, Jacopo Forneris, Federico Picollo, Valentina Carabelli, Paolo Olivero, Alfio Battiato, Laura Guarina, Andrea Marcantoni, Emilio Carbone, Daniela Gavello, Marco Genovese, Ekaterina Moreva, Marta Gai, Paolo Traina |
| المصدر: | Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018) Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Postsynaptic Current, Somatic cell, Action Potentials, FOS: Physical sciences, lcsh:Medicine, 02 engineering and technology, Hippocampal formation, Inhibitory postsynaptic potential, Hippocampus, Models, Biological, Article, Nanodiamonds, 03 medical and health sciences, Mice, Animals, Physics - Biological Physics, lcsh:Science, Cells, Cultured, Neurons, Multidisciplinary, Chemistry, lcsh:R, 021001 nanoscience & nanotechnology, Photobleaching, Microelectrode, Electrophysiology, 030104 developmental biology, Biological Physics (physics.bio-ph), Quantitative Biology - Neurons and Cognition, FOS: Biological sciences, Excitatory postsynaptic potential, Biophysics, Neurons and Cognition (q-bio.NC), lcsh:Q, Nerve Net, 0210 nano-technology |
| الوصف: | Fluorescent nanodiamonds (FND) are carbon-based nanomaterials that can efficiently incorporate optically active photoluminescent centers such as the nitrogen-vacancy complex, thus making them promising candidates as optical biolabels and drug-delivery agents. FNDs exhibit bright fluorescence without photobleaching combined with high uptake rate and low cytotoxicity. Focusing on FNDs interference with neuronal function, here we examined their effect on cultured hippocampal neurons, monitoring the whole network development as well as the electrophysiological properties of single neurons. We observed that FNDs drastically decreased the frequency of inhibitory (from 1.81 Hz to 0.86 Hz) and excitatory (from 1.61 Hz to 0.68 Hz) miniature postsynaptic currents, and consistently reduced action potential (AP) firing frequency (by 36%), as measured by microelectrode arrays. On the contrary, bursts synchronization was preserved, as well as the amplitude of spontaneous inhibitory and excitatory events. Current-clamp recordings revealed that the ratio of neurons responding with AP trains of high-frequency (fast-spiking) versus neurons responding with trains of low-frequency (slow-spiking) was unaltered, suggesting that FNDs exerted a comparable action on neuronal subpopulations. At the single cell level, rapid onset of the somatic AP ("kink") was drastically reduced in FND-treated neurons, suggesting a reduced contribution of axonal and dendritic components while preserving neuronal excitability. Comment: 34 pages, 9 figures |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb1d8471d8f0c84c794f78b8cc1a936b http://link.springer.com/article/10.1038/s41598-018-20528-5 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....eb1d8471d8f0c84c794f78b8cc1a936b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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