Polymer-coated bioactive glass S53P4 increases VEGF and TNF expression in an induced membrane model in vivo
| العنوان: | Polymer-coated bioactive glass S53P4 increases VEGF and TNF expression in an induced membrane model in vivo |
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| المؤلفون: | Jukka Pajarinen, Gustav Strömberg, Leena Hupa, Mari Ainola, Tom Böhling, Nina Lindfors, Peter Uppstu, Robert Björkenheim |
| المساهمون: | Clinicum, Department of Surgery, Plastiikkakirurgian yksikkö, University of Helsinki, HUSLAB, Department of Pathology, Tom Böhling / Principal Investigator, Medicum, HUS Internal Medicine and Rehabilitation, HUS Musculoskeletal and Plastic Surgery |
| المصدر: | Journal of Materials Science. 52:9055-9065 |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Materials science, 0206 medical engineering, macromolecular substances, 02 engineering and technology, BONE REGENERATION, chemistry.chemical_compound, In vivo, General Materials Science, Methyl methacrylate, GROWTH-FACTORS, REPAIR, 318 Medical biotechnology, Mechanical Engineering, technology, industry, and agriculture, 3126 Surgery, anesthesiology, intensive care, radiology, 021001 nanoscience & nanotechnology, 020601 biomedical engineering, FRACTURE, FIBROBLAST, Vascular endothelial growth factor, PLGA, Real-time polymerase chain reaction, Membrane, chemistry, Mechanics of Materials, CELLS, STIMULATE ANGIOGENESIS, Tumor necrosis factor alpha, Implant, 0210 nano-technology, Biomedical engineering |
| الوصف: | The two-stage induced-membrane technique for treatment of large bone defects has become popular among orthopedic surgeons. In the first operation, the bone defect is filled with poly(methyl methacrylate) (PMMA), which is intended to produce a membrane around the implant. In the second operation, PMMA is replaced with autograft or allograft bone. Bioactive glasses (BAGs) are bone substitutes with bone-stimulating and angiogenetic properties. The aim of our study was to evaluate the inductive vascular capacity of BAG-S53P4 and poly(lactide-co-glycolide) (PLGA)-coated BAG-S53P4 for potential use as bone substitutes in a single-stage induced-membrane technique. Sintered porous rods of BAG-S53P4, PLGA-coated BAG-S53P4 and PMMA were implanted in the femur of 36 rabbits for 2, 4 and 8 weeks. The expression of vascular endothelial growth factor (VEGF) and tumor necrosis factor alpha (TNF) in the induced membranes of implanted materials was analyzed with real-time quantitative polymerase chain reaction and compared with histology. Both uncoated BAG-S53P4 and PLGA-coated BAG-S53P4 increase expression of VEGF and TNF, resulting in higher amounts of capillary beds, compared with the lower expression of VEGF and less capillary beads observed for negative control and PMMA samples. A significantly higher expression of VEGF was observed for PLGA-coated BAG-S53P4 than for PMMA at 8 weeks (p |
| تدمد: | 1573-4803 0022-2461 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb43ccc2b1e1e518c332ffae3a324eb0 https://doi.org/10.1007/s10853-017-0839-6 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....eb43ccc2b1e1e518c332ffae3a324eb0 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15734803 00222461 |
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