cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo
| العنوان: | cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo |
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| المؤلفون: | Clemens van Blitterswijk, Anouk Leusink, Riccardo Fodde, Joyce Doorn, Frank Janssen, Cristina Olivo, Jan de Boer, Linda van Rijn, Ramakrishnaiah Siddappa, Claudia Gaspar, Ruud Licht, Anton C.M. Martens |
| المساهمون: | Faculty of Science and Technology, University of Groningen, Hematology, Pathology, Public Health, Pediatrics |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America, 105(20), 7281-7286. National Academy of Sciences Proceedings of the National Academy of Sciences of the United States of America, 105(20), 7281-7286. NATL ACAD SCIENCES Proceedings of the National Academy of Sciences of the United States of America, 105(20), 7281 Proceedings of the National Academy of Sciences of the U.S.A., 105(20), 7281-7286. National Academy of Sciences |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | medicine.medical_specialty, OSTEOGENIC DIFFERENTIATION, Stromal cell, Bone Morphogenetic Protein 2, TELOMERASE EXPRESSION, Biology, METIS-253659, Bone morphogenetic protein, Models, Biological, Bone morphogenetic protein 2, Bone and Bones, osteogenesis, OSTEOBLAST DIFFERENTIATION, MECHANISMS, Tissue engineering, TISSUE-ENGINEERED BONE, Transforming Growth Factor beta, In vivo, Internal medicine, Cyclic AMP, medicine, Humans, Insulin-Like Growth Factor I, Protein kinase A, bone tissue engineering, IR-60305, Inhibitor of Differentiation Protein 2, Multidisciplinary, PKA signaling, Tissue Engineering, GENE-TRANSCRIPTION, MARROW STROMAL CELLS, Mesenchymal stem cell, PROLIFERATION, Mesenchymal Stem Cells, Transforming growth factor beta, Biological Sciences, PROTEIN-KINASE, Interleukin-11, Cyclic AMP-Dependent Protein Kinases, Cell biology, Endocrinology, Bone Morphogenetic Proteins, biology.protein, Cytokines, SIGNALING PATHWAY, Proto-Oncogene Proteins c-fos, Signal Transduction |
| الوصف: | Tissue engineering of large bone defects is approached through implantation of autologous osteogenic cells, generally referred to as multipotent stromal cells or mesenchymal stem cells (MSCs). Animal-derived MSCs successfully bridge large bone defects, but models for ectopic bone formation as well as recent clinical trials demonstrate that bone formation by human MSCs (hMSCs) is inadequate. The expansion phase presents an attractive window to direct hMSCs by pharmacological manipulation, even though no profound effect on bone formation in vivo has been described so far using this approach. We report that activation of protein kinase A elicits an immediate response through induction of genes such as ID2 and FosB , followed by sustained secretion of bone-related cytokines such as BMP-2, IGF-1, and IL-11. As a consequence, PKA activation results in robust in vivo bone formation by hMSCs derived from orthopedic patients. |
| وصف الملف: | application/pdf; image/pdf |
| اللغة: | English |
| تدمد: | 0027-8424 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb6249cd8cab91110445b2860d750263 https://research.utwente.nl/en/publications/18d546f7-ef48-46ad-8740-dd9ab3c76329 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....eb6249cd8cab91110445b2860d750263 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00278424 |
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