The Effect of Cold Atmospheric Plasma on the Membrane Permeability of Human Osteosarcoma Cells
| العنوان: | The Effect of Cold Atmospheric Plasma on the Membrane Permeability of Human Osteosarcoma Cells |
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| المؤلفون: | Matthias B. Stope, Rebekka Einenkel, Damián O. Muzzio, Lyubomir Haralambiev, Denis Gümbel, Axel Ekkernkamp, Martin Burchardt, Andreas Nitsch, Marek Zygmunt, Nadine Gelbrich |
| المصدر: | Anticancer Research. 40:841-846 |
| بيانات النشر: | Anticancer Research USA Inc., 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Osteosarcoma, Cancer Research, Cell Membrane Permeability, Plasma Gases, Membrane permeability, Cell growth, Chemistry, Cell, Motility, General Medicine, Cell biology, 03 medical and health sciences, 0302 clinical medicine, medicine.anatomical_structure, Oncology, Cell culture, Apoptosis, Cell Line, Tumor, 030220 oncology & carcinogenesis, Cancer cell, medicine, Humans, Secretion |
| الوصف: | Background Cold atmospheric plasma (CAP) has a variety of anticancer effects on different cancer cell types. In osteosarcoma (OS) cells, CAP reduces growth and motility, induces apoptosis, and alters secretion of cellular factors. The influence of CAP on membrane integrity of OS cells is unknown. Materials and methods Two different OS cell lines (U-2 OS and MNNG-HOS) were treated with CAP. Proliferation assays for cell growth after treatment was performed. Alterations in membrane permeability and the associated translocation of low molecular weight particles through the cytoplasmic membrane of OS cells after CAP treatment were shown in fluorescein diacetate (FDA) assays. Results FDA increasingly passed the membrane after CAP treatment and this effect depended on the duration of treatment. It was also shown that after CAP treatment, FDA was able to diffuse into the cells from the outside as well as out of the cell interior. These effects were observed when CAP-treated buffer was used and therefore no direct contact between cells and CAP occurred. Conclusion The observations suggest that changes in membrane permeability and function may contribute to the antiproliferative effects of CAP. |
| تدمد: | 1791-7530 0250-7005 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb96958e9b34b444fad1dfd336bb4c25 https://doi.org/10.21873/anticanres.14016 |
| رقم الأكسشن: | edsair.doi.dedup.....eb96958e9b34b444fad1dfd336bb4c25 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17917530 02507005 |
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