The RY/Sph element mediates transcriptional repression of maturation genes from late maturation to early seedling growth
| العنوان: | The RY/Sph element mediates transcriptional repression of maturation genes from late maturation to early seedling growth |
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| المؤلفون: | Nathalie Martin, Anna Golovko, Ines Ezcurra, Lars Rask, Gea Guerriero, Jens F. Sundström |
| المصدر: | New Phytologist. 184:552-565 |
| بيانات النشر: | Wiley, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Transcriptional Activation, DNA, Plant, Physiology, Arabidopsis, Repressor, Plant Science, Biology, Genes, Plant, Models, Biological, Transactivation, Gene Expression Regulation, Plant, Genes, Reporter, Transcription (biology), Tobacco, Gene expression, Phosphoprotein Phosphatases, Gene, Psychological repression, Regulation of gene expression, Reporter gene, Base Sequence, Brassica napus, Gene Expression Regulation, Developmental, Plants, Genetically Modified, Staurosporine, Molecular biology, Protein Phosphatase 2C, Seedlings, Seeds |
| الوصف: | In orthodox seeds, the transcriptional activator ABI3 regulates two major stages in embryo maturation: a mid-maturation (MAT) stage leading to accumulation of storage compounds, and a late maturation (LEA) stage leading to quiescence and desiccation tolerance. Our aim was to elucidate mechanisms for transcriptional shutdown of MAT genes during late maturation, to better understand phase transition between MAT and LEA stages. Using transgenic and transient approaches in Nicotiana, we examined activities of two ABI3-dependent reporter genes driven by multimeric RY and abscisic acid response elements (ABREs) from a Brassica napus napin gene, termed RY and ABRE, where the RY reporter requires ABI3 DNA binding. Expression of RY peaks during mid-maturation and drops during late maturation, mimicking the MAT gene program, and in Arabidopsis thaliana RY elements are over-represented in MAT, but not in LEA, genes. The ABI3 transactivation of RY is inhibited by staurosporine, by a PP2C phosphatase, and by a repressor of maturation genes, VAL1/HSI2. The RY element mediates repression of MAT genes, and we propose that transcriptional shutdown of the MAT program during late maturation involves inhibition of ABI3 DNA binding by dephosphorylation. Later, during seedling growth, VAL1/HSI2 family repressors silence MAT genes by binding RY elements. |
| تدمد: | 1469-8137 0028-646X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb96e613b733b49f3f3f469d8b2a3902 https://doi.org/10.1111/j.1469-8137.2009.02977.x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....eb96e613b733b49f3f3f469d8b2a3902 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14698137 0028646X |
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