Whole-Genome Sequencing of 117 Chromosome Segment Substitution Lines for Genetic Analyses of Complex Traits in Rice
| العنوان: | Whole-Genome Sequencing of 117 Chromosome Segment Substitution Lines for Genetic Analyses of Complex Traits in Rice |
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| المؤلفون: | Jiongjiong Fan, Hua Hua, Zhaowei Luo, Qi Zhang, Mengjiao Chen, Junyi Gong, Xin Wei, Zonghua Huang, Xuehui Huang, Qin Wang |
| المصدر: | Rice Rice, Vol 15, Iss 1, Pp 1-11 (2022) |
| بيانات النشر: | Springer US, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | QTL mapping, RFT1, Chromosome segment substitution lines (CSSLs), Heading date, Soil Science, Plant culture, food and beverages, Original Article, Plant Science, Rice, Agronomy and Crop Science, SB1-1110 |
| الوصف: | Rice is one of the most important food crops in Asia. Genetic analyses of complex traits and molecular breeding studies in rice greatly rely on the construction of various genetic populations. Chromosome segment substitution lines (CSSLs) serve as a powerful genetic population for quantitative trait locus (QTL) mapping in rice. Moreover, CSSLs containing target genomic regions can be used as improved varieties in rice breeding. In this study, we developed a set of CSSLs consisting of 117 lines derived from the recipient ‘Huanghuazhan’ (HHZ) and the donor ‘Basmati Surkb 89–15’ (BAS). The 117 lines were extensively genotyped by whole-genome resequencing, and a high-density genotype map was constructed for the CSSL population. The 117 CSSLs covered 99.78% of the BAS genome. Each line contained a single segment, and the average segment length was 6.02 Mb. Using the CSSL population, we investigated three agronomic traits in Shanghai and Hangzhou, China, and a total of 25 QTLs were detected in both environments. Among those QTLs, we found that RFT1 was the causal gene for heading date variance between HHZ and BAS. RFT1 from BAS was found to contain a loss-of-function allele based on yeast two-hybrid assay, and its causal variation was a P to S change in the 94th amino acid of the RFT1 protein. The combination of high-throughput genotyping and marker-assisted selection (MAS) is a highly efficient way to construct CSSLs in rice, and extensively genotyped CSSLs will be a powerful tool for the genetic mapping of agronomic traits and molecular breeding for target QTLs/genes. Supplementary Information The online version contains supplementary material available at 10.1186/s12284-022-00550-y. |
| اللغة: | English |
| تدمد: | 1939-8433 1939-8425 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebfcd5aacf6714282eb8e073f3d24c4d http://europepmc.org/articles/PMC8758858 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ebfcd5aacf6714282eb8e073f3d24c4d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19398433 19398425 |
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