Ligation-Mediated Polymerase Chain Reaction Detection of 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine and 5-Hydroxycytosine at the Codon 176 of the p53 Gene of Hepatitis C-Associated Hepatocellular Carcinoma Patients
| العنوان: | Ligation-Mediated Polymerase Chain Reaction Detection of 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine and 5-Hydroxycytosine at the Codon 176 of the p53 Gene of Hepatitis C-Associated Hepatocellular Carcinoma Patients |
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| المؤلفون: | Roger W. Giese, Mirko Tarocchi, Filippo Cellai, Roger W. L. Godschalk, Elisabetta Ceni, Marco Peluso, Andrea Galli, Armelle Munnia, Frederik J. van Schooten |
| المساهمون: | Farmacologie en Toxicologie, RS: NUTRIM - R3 - Respiratory & Age-related Health |
| المصدر: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 21, Iss 6753, p 6753 (2020) Volume 21 Issue 18 International Journal of Molecular Sciences, 21(18):6753. Multidisciplinary Digital Publishing Institute (MDPI) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, p53, PATHOGENESIS, Hepacivirus, medicine.disease_cause, Polymerase Chain Reaction, law.invention, Lipid peroxidation, lcsh:Chemistry, chemistry.chemical_compound, DNA Adducts, 0302 clinical medicine, law, Deoxyguanosine, heterocyclic compounds, TP53, HCC, lcsh:QH301-705.5, Spectroscopy, Polymerase chain reaction, Chemistry, Liver Neoplasms, METHYLATION, General Medicine, Hepatitis C, Hep G2 Cells, Computer Science Applications, 8-Hydroxy-2'-Deoxyguanosine, 030220 oncology & carcinogenesis, Hepatocellular carcinoma, HCV, malondialdehyde, Carcinoma, Hepatocellular, Hepatitis C virus, M1dG, 8-oxodG, ros, Context (language use), oxidative dna-damage, Catalysis, Article, Inorganic Chemistry, 03 medical and health sciences, Cytosine, ADDUCTS, INFLAMMATION, Cell Line, Tumor, medicine, cancer, Humans, Physical and Theoretical Chemistry, Codon, m(1)dg, Molecular Biology, Gene, Organic Chemistry, SOMATIC MUTATIONS, medicine.disease, Genes, p53, Molecular biology, digestive system diseases, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, Lipid Peroxidation, Reactive Oxygen Species |
| الوصف: | Molecular mechanisms underlying Hepatitis C virus (HCV)-associated hepatocellular carcinoma (HCC) pathogenesis are still unclear. Therefore, we analyzed the levels of 8-oxo-7,8-dihydro-2&prime deoxyguanosine (8-oxodG) and other oxidative lesions at codon 176 of the p53 gene, as well as the generation of 3-(2-deoxy-&beta d-erythro-pentafuranosyl)pyrimido[1,2-&alpha ]purin-10(3H)-one deoxyguanosine (M1dG), in a cohort of HCV-related HCC patients from Italy. Detection of 8-oxodG and 5-hydroxycytosine (5-OHC) was performed by ligation mediated-polymerase chain reaction assay, whereas the levels of M1dG were measured by chromatography and mass-spectrometry. Results indicated a significant 130% excess of 8-oxodG at &ndash TGC&ndash position of p53 codon 176 in HCV-HCC cases as compared to controls, after correction for age and gender, whereas a not significant increment of 5-OHC at &ndash position was found. Then, regression models showed an 87% significant excess of M1dG in HCV-HCC cases relative to controls. Our study provides evidence that increased adduct binding does not occur randomly on the sequence of the p53 gene but at specific sequence context in HCV-HCC patients. By-products of lipid peroxidation could also yield a role in HCV-HCC development. Results emphasize the importance of active oxygen species in inducing nucleotide lesions at a p53 mutational hotspot in HCV-HCC patients living in geographical areas without dietary exposure to aflatoxin B1. |
| وصف الملف: | application/pdf |
| تدمد: | 1422-0067 1661-6596 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec1e9010247af91eb2a534b2753b7308 https://pubmed.ncbi.nlm.nih.gov/32942546 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ec1e9010247af91eb2a534b2753b7308 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 16616596 |
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