Analysis of killing of growing cells and dormant and germinated spores of Bacillus species by black silicon nanopillars
| العنوان: | Analysis of killing of growing cells and dormant and germinated spores of Bacillus species by black silicon nanopillars |
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| المؤلفون: | Priya Vashishta, Peter Setlow, Jayakanth Ravichandran, Shanyuan Niu, Aiichiro Nakano, Stephen M. King, Maya Yankova, Matthew Mecklenburg, Sonali Ghosh, Rajiv K. Kalia |
| المصدر: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
| بيانات النشر: | Nature Publishing Group UK, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Bacillus species, Multidisciplinary, biology, Chemistry, lcsh:R, 030106 microbiology, Black silicon, fungi, Bacillus cereus, lcsh:Medicine, biology.organism_classification, Bactericidal effect, bacterial infections and mycoses, Article, Microbiology, Spore, 03 medical and health sciences, chemistry.chemical_compound, Germination, lcsh:Q, lcsh:Science, human activities, Bacillus megaterium, Nanopillar |
| الوصف: | Black silicon (bSi) wafers with a high density of high-aspect ratio nanopillars have recently been suggested to have mechanical bactericidal activity. However, it remains unclear whether bSi with the nanopillars can kill only growing bacterial cells or also dormant spores that are harder to kill. We have reexamined the cidal activity of bSi on growing cells, dormant and germinated spores of B. subtilis, and dormant spores of several other Bacillus species by incubation on bSi wafers with and without nanopillars. We found that the bSi wafers with nanopillars were indeed very effective in rupturing and killing the growing bacterial cells, while wafers without nanopillars had no bactericidal effect. However, bSi wafers with or without nanopillars gave no killing or rupture of dormant spores of B. subtilis, Bacillus cereus or Bacillus megaterium, although germinated B. subtilis spores were rapidly killed. This work lays a foundation for novel bactericidal applications of bSi by elucidating the limits of mechanical bactericidal approaches. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec310c9dacb3b23e0c9c05c5186799c3 http://europepmc.org/articles/PMC5736721 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ec310c9dacb3b23e0c9c05c5186799c3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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