Process optimization for the rapid production of Enterovirus 71
| العنوان: | Process optimization for the rapid production of Enterovirus 71 |
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| المؤلفون: | Lan-Lan Xiao, Ke-Da Chen, Da-Zhi Chen, Zhang Yanjun, Kaizhou Huang, Xiaoxin Wu, Lanjuan Li |
| المصدر: | Cytotechnology |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Chromatography, biology, Chemistry, Clinical Biochemistry, Biomedical Engineering, Bioengineering, Cell Biology, biology.organism_classification, Virus, 03 medical and health sciences, Titer, 030104 developmental biology, 0302 clinical medicine, Multiplicity of infection, 030220 oncology & carcinogenesis, Vero cell, Enterovirus 71, Original Article, Process optimization, Seeding, Epidemic strain, Biotechnology |
| الوصف: | Enterovirus 71 (EV71) infection can cause hand–foot-and-mouth disease (HFMD). Inactivated EV71 vaccine was effective to prevent EV71 derived HFMD. A highly efficient and economical process for producing EV71 is needed. In our study, the epidemic strain of EV71 (EV71-2013ZJHFMD) was obtained and purified. The Vero cells were cultured for production of EV71. The mini-bioreactor vessel (Amprotein Inc., China) packed with a 0.6 g polymer fiber carrier was used to determine the best seeding cell density, multiplicity of infection (MOI) and temperature. Then the optimized procedure was further applied in a 10 L disposable perfusion bioreactor ACPB (AmProtein Current Perfusion Bioreactor). The Vero cell culture and viral titer were monitored. The seeding density of 1.5 × 10(7) cells per 0.6 g disk was considered to be the most appropriate for the culture. The best MOI was 0.1 and the temperature was 32 °C. The total cell number increased from 1.5 × 10(9) to 3.0 × 10(10). The maximum viral titers reached 1.0 × 10(8)/mL 3 days post-infection in our optimized special culture procedure (serum-free during the harvest period, supplemented with 0.25% Lactalbumin Hydrolysate). The total volume of the harvested supernatant was 25 L and the total virus yield was 1.93 × 10(12). The procedure using Vero cells grown on polymer fiber paper carriers was effective for the large-scale production of EV71. |
| تدمد: | 1573-0778 0920-9069 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ecca1ab5ee3aae70c3e263de146692e4 https://doi.org/10.1007/s10616-019-00340-3 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ecca1ab5ee3aae70c3e263de146692e4 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15730778 09209069 |
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