Glutamate Increases Glycogen Content and Reduces Glucose Utilization in Primary Astrocyte Culture
| العنوان: | Glutamate Increases Glycogen Content and Reduces Glucose Utilization in Primary Astrocyte Culture |
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| المؤلفون: | Raymond A. Swanson, Albert C. H. Yu, Frank R. Sharp, Pak H. Chan |
| المصدر: | Journal of Neurochemistry. 54:490-496 |
| بيانات النشر: | Wiley, 1990. |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | medicine.medical_specialty, Glucose-6-Phosphate, Glutamic Acid, Biochemistry, Cellular and Molecular Neuroscience, chemistry.chemical_compound, Glutamates, Internal medicine, Glycogen branching enzyme, medicine, Animals, Glutamate receptor antagonist, Cells, Cultured, Glycogen, biology, Glucosephosphates, Glutamate receptor, Glutamic acid, Biomechanical Phenomena, Receptors, Neurotransmitter, Glucose, medicine.anatomical_structure, Endocrinology, Receptors, Glutamate, Glucose 6-phosphate, chemistry, Glycogenesis, Astrocytes, biology.protein, Astrocyte |
| الوصف: | The glycogen content of primary cultured astrocytes was approximately doubled by incubation with 1 mM L-glutamate or L-aspartate. Other amino acids and excitatory neurotransmitters were without effect. The increase in glycogen level was not blocked by the glutamate receptor antagonist kynurenic acid but was completely blocked by the glutamate uptake inhibitor threo-3-hydroxy-D,L-aspartate and by removal of Na+ from the medium. Incubation with radiolabeled glucose and glutamate revealed that the increased glycogen content was derived almost entirely from glucose. Glutamate at 1 mM was also found to cause a 53 +/- 12% decrease in glucose utilization and a 112 +/- 69% increase in glucose-6-phosphate levels. These results suggest that the glycogen content of astrocytes is linked to the rate of glucose utilization and that glucose utilization can, in turn, be affected by the availability of alternative metabolic substrates. These relationships suggest a mechanism by which brain glycogen accumulation occurs during decreased neuronal activity. |
| تدمد: | 1471-4159 0022-3042 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ecd589e97d052dc6950a841bd87d7b26 https://doi.org/10.1111/j.1471-4159.1990.tb01898.x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....ecd589e97d052dc6950a841bd87d7b26 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14714159 00223042 |
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