Differential Expression of Super-Enhancer-Associated Long Non-coding RNAs in Uterine Leiomyomas
| العنوان: | Differential Expression of Super-Enhancer-Associated Long Non-coding RNAs in Uterine Leiomyomas |
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| المؤلفون: | Tsai-Der Chuang, Derek Quintanilla, Drake Boos, Omid Khorram |
| المصدر: | Reproductive Sciences. 29:2960-2976 |
| بيانات النشر: | Springer Science and Business Media LLC, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Ligases, Leiomyoma, Mutation, Myometrium, Humans, Polycomb-Group Proteins, Obstetrics and Gynecology, Female, RNA, Long Noncoding, RNA, Messenger |
| الوصف: | Super-enhancer-associated long non-coding RNAs (SE-lncRNAs) are a specific set of lncRNAs transcribed from super-enhancer (SE) genomic regions. Recent studies have revealed that SE-lncRNAs play essential roles in tumorigenesis through the regulation of oncogenes. The objective of this study was to elucidate the expression profile of SE-lncRNAs with concurrent assessment of associated mRNAs in leiomyomas and paired myometrium. Arraystar SE-lncRNAs arrays were used to systematically profile the differentially expressed SE-lncRNAs along with the corresponding SE-regulated protein coding genes in eight leiomyomas and paired myometrium. The analysis indicated 7680 SE-lncRNAs were expressed, of which 721 SE-lncRNAs were overexpressed, while 247 SE-lncRNAs were underexpressed by 1.5-fold or greater in leiomyoma. Thirteen novel SE-lncRNAs and their corresponding protein coding genes were selected, and their expression was confirmed in eighty-one paired leiomyoma tissues by quantitative real-time PCR. The thirteen pairs of SE-lncRNAs and their corresponding protein coding genes included RP11-353N14.2/CBX4, SOCS2-AS1/SOCS2, RP1-170O19.14/HOXA11, CASC15/PRL, EGFLAM-AS1/EGFLAM, RP11-225H22/NEURL1, RP5-1086K13.1/CD58, AC092839.3/SPTBN1, RP11-69I8.3/CTGF, TM4SF1-AS1/TM4SF1, RP11-373D23/FOSL2, RP11-399K21.11/COMTD1, and CTB-113P19.1/SPARC. Among these SE-lncRNAs, the expression of SOCS2-AS1/SOCS2, RP11-353N14.2/CBX4, RP1-170O19.14/HOXA11, and RP11-225H22/NEURL1 was significantly higher in African Americans as compared with Caucasians. The expression of RP11-353N14.2/CBX4, SOCS2-AS1/SOCS2, CASC15/PRL, and CTB-113P19.1/SPARC was significantly higher in tumors with MED12-mutation-positive as compared with MED12-mutation-negative tumors. Collectively, our results indicate that the differential expression of SE in leiomyomas is another mechanism contributing to dysregulation of protein coding genes in leiomyomas and that race and MED12 mutation can influence the expression of a select group of SE. |
| تدمد: | 1933-7205 1933-7191 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed78c51ac238d484e9737f30e9d4636c https://doi.org/10.1007/s43032-022-00981-4 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ed78c51ac238d484e9737f30e9d4636c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19337205 19337191 |
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