Characterization and validation of olive FAD and SAD gene families: expression analysis in different tissues and during fruit development
العنوان: | Characterization and validation of olive FAD and SAD gene families: expression analysis in different tissues and during fruit development |
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المؤلفون: | Mariela Torres, Soraya Mousavi, L. Gentili, M. Tivani, C Guerrero, Roberto Mariotti, Pierluigi Pierantozzi, L Roka, Cibeles Contreras, Damian Maestri, Luciana Baldoni, Nicolò G. M. Cultrera |
المصدر: | Molecular biology reports 47 (2020): 4345–4355. doi:10.1007/s11033-020-05554-9 info:cnr-pdr/source/autori:Contreras C.; Mariotti R.; Mousavi S.; Baldoni L.; Guerrero C.; Roka L.; Cultrera N.; Pierantozzi P.; Maestri D.; Gentili L.; Tivani M.; Torres M./titolo:Characterization and validation of olive FAD and SAD gene families: expression analysis in different tissues and during fruit development/doi:10.1007%2Fs11033-020-05554-9/rivista:Molecular biology reports/anno:2020/pagina_da:4345/pagina_a:4355/intervallo_pagine:4345–4355/volume:47 |
بيانات النشر: | Springer Science and Business Media LLC, 2020. |
سنة النشر: | 2020 |
مصطلحات موضوعية: | Fatty Acid Desaturases, Candidate gene, Biology, Genes, Plant, Mixed Function Oxygenases, chemistry.chemical_compound, Protein structure, Plant Growth Regulators, Gene Expression Regulation, Plant, Olea, Gene expression, Genetics, Gene family, Molecular Biology, Gene, Plant Proteins, Gene Expression Profiling, Fatty Acids, food and beverages, Ripening, Lipid metabolism, General Medicine, Olive (olea europaea · Subsp. europaea · Var. europaea) · SAD · FAD · Gene expression · RT qPCR · Fruit mesocarp, Oleic acid, Biochemistry, chemistry, Fruit, Fatty Acids, Unsaturated, Oleic Acid |
الوصف: | Stearoyl-ACP desaturases (SADs) and fatty acid desaturases (FADs) play a critical role in plant lipid metabolism and also affect oil fatty acid composition introducing double bonds into the hydrocarbon chains to produce unsaturated fatty acids. In the present study, the genomic sequences of three SAD and three FAD candidate genes were characterized in olive and their expression was evaluated in different plant tissues. OeSAD genes corresponded to olive SAD1 and SAD2 and to a newly identified OeSAD4, sharing the conserved protein structure with other plant species. On the other hand, the full-length genomic sequences of two microsomal OeFAD genes (FAD2-1 and FAD2-2) and the plastidial FAD6, were released. When the level of expression was tested on different tissues of cv. Leccino, OeSAD1 and OeSAD2 were mainly expressed in the fruits, while OeFAD genes showed the lowest expression in this tissue. The mRNA profiling of all genes was directly studied in fruits of Leccino and Coratina cultivars during fruit development. In both genotypes, the expression level of OeSAD1 and OeSAD2 had the highest value during and after the pit-hardening period, when oil accumulation in fruit mesocarp is intensively increasing. Furthermore, the expression level of both OeFAD2 genes, which were the main candidates for oleic acid desaturation, were almost negligible during fruit ripening. These results have made possible to define candidate genes of the machinery regulation of fatty acid composition in olive oil, providing information on their sequence, gene structure and chromosomal location. |
تدمد: | 1573-4978 0301-4851 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed8545614f56cbe9a87f919dc4d18ae7 https://doi.org/10.1007/s11033-020-05554-9 |
حقوق: | CLOSED |
رقم الأكسشن: | edsair.doi.dedup.....ed8545614f56cbe9a87f919dc4d18ae7 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 15734978 03014851 |
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