In Vivo Force Application Reveals a Fast Tissue Softening and External Friction Increase during Early Embryogenesis
العنوان: | In Vivo Force Application Reveals a Fast Tissue Softening and External Friction Increase during Early Embryogenesis |
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المؤلفون: | Guillaume Salbreux, Jérôme Solon, Kai Dierkes, Carlo Carolis, Arturo D’Angelo |
المصدر: | Recercat. Dipósit de la Recerca de Catalunya instname |
بيانات النشر: | Cell Press, 2019. |
سنة النشر: | 2019 |
مصطلحات موضوعية: | 0301 basic medicine, Early embryogenesis, Embryo, Nonmammalian, Embriologia, Library science, Embryonic Development, Biology, General Biochemistry, Genetics and Molecular Biology, Article, Biomechanical Phenomena, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Drosophila melanogaster, Animals, Christian ministry, Tissue mechanics, General Agricultural and Biological Sciences, Genètica, 030217 neurology & neurosurgery, Cytoskeleton, Structural Biology & Biophysics, Computational & Systems Biology |
الوصف: | During development, cell-generated forces induce tissue-scale deformations to shape the organism [1,2]. The pattern and extent of these deformations depend not solely on the temporal and spatial profile of the generated force fields but also on the mechanical properties of the tissues that the forces act on. It is thus conceivable that, much like the cell-generated forces, the mechanical properties of tissues are modulated during development in order to drive morphogenesis toward specific developmental endpoints. Although many approaches have recently emerged to assess effective mechanical parameters of tissues [3-8], they could not quantitatively relate spatially localized force induction to tissue-scale deformations in vivo. Here, we present a method that overcomes this limitation. Our approach is based on the application of controlled forces on a single microparticle embedded in an individual cell of an embryo. Combining measurements of bead displacement with the analysis of induced deformation fields in a continuum mechanics framework, we quantify material properties of the tissue and follow their changes over time. In particular, we uncover a rapid change in tissue response occurring during Drosophila cellularization, resulting from a softening of the blastoderm and an increase of external friction. We find that the microtubule cytoskeleton is a major contributor to epithelial mechanics at this stage. We identify developmentally controlled modulations in perivitelline spacing that can account for the changes in friction. Overall, our method allows for the measurement of key mechanical parameters governing tissue-scale deformations and flows occurring during morphogenesis. The research leading to these results has received funding from the Spanish Ministry of Economy and Competitiveness (MEIC) to the EMBL partnership, Plan Nacional, BFU2010-16546 and BFU2015-68754, and “Centro de Excelencia Severo Ochoa 2013–2017,” SEV-2012-0208. We acknowledge the support of the CERCA Programme/Generalitat de Catalunya. G.S. is supported by the Francis Crick Institute, which receives its core funding from Cancer Research UK (FC001317), UK Medical Research Council (FC001317), and Wellcome Trust (FC001317) |
وصف الملف: | application/pdf |
اللغة: | English |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::edafe558cc0b07eff45f65223efbdc69 https://europepmc.org/articles/PMC6509404/ |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....edafe558cc0b07eff45f65223efbdc69 |
قاعدة البيانات: | OpenAIRE |
الوصف غير متاح. |