SSR Markers for Trichoderma virens: Their Evaluation and Application to Identify and Quantify Root-Endophytic Strains
| العنوان: | SSR Markers for Trichoderma virens: Their Evaluation and Application to Identify and Quantify Root-Endophytic Strains |
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| المؤلفون: | Jessica Zwanzig, Ingo Schellenberg, Sophie Heckendorff, Joerg Geistlinger |
| المصدر: | Diversity, Vol 7, Iss 4, Pp 360-384 (2015) Diversity Volume 7 Issue 4 Pages 360-384 |
| بيانات النشر: | MDPI AG, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | microsatellite, Locus (genetics), Fungus, strain identification, Biology, law.invention, 03 medical and health sciences, chemistry.chemical_compound, simple sequence repeat, law, Molecular marker, Botany, biocontrol, lcsh:QH301-705.5, Polymerase chain reaction, fragment analysis, 030304 developmental biology, Nature and Landscape Conservation, molecular marker, 2. Zero hunger, 0303 health sciences, Rhizosphere, Ecology, 030306 microbiology, Inoculation, Ecological Modeling, Hypocrea virens, biology.organism_classification, Agricultural and Biological Sciences (miscellaneous), qPCR, monitoring, lcsh:Biology (General), chemistry, Genetic distance, root endophyte, Microsatellite |
| الوصف: | Using biological fertilizers and pesticides based on beneficial soil microbes in order to reduce mineral fertilizers and chemical pesticides in conventional agriculture is still a matter of debate. In this regard, a European research project seeks to elucidate the role of root-endophytic fungi and to develop molecular tools to trace and quantify these fungi in the rhizosphere and root tissue. To do this, the draft genome sequence of the biocontrol fungus Trichoderma virens (T. virens) was screened for simple sequence repeats (SSRs) and primers were developed for 12 distinct loci. Primers were evaluated using a global collection of ten isolates where an average of 7.42 alleles per locus was detected. Nei’s standard genetic distance ranged from 0.18 to 0.27 among the isolates, and the grand mean of haploid diversity in AMOVA analysis was 0.693 ± 0.019. Roots of tomato plants were inoculated with different strains and harvested six weeks later. Subsequent PCR amplification identified root-endophytic strains and co-colonization of roots by different strains. Markers were applied to qPCR to quantify T. virens strains in root tissue and to determine their identity using allele-specific melting curve analysis. Thus, the root-endophytic lifestyle of T. virens was confirmed, strains in roots were quantified and simultaneous colonization of roots by different strains was observed. |
| وصف الملف: | application/pdf |
| تدمد: | 1424-2818 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::edc1e275e0c7292d07d76e7cff772d46 https://doi.org/10.3390/d7040360 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....edc1e275e0c7292d07d76e7cff772d46 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14242818 |
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