OTX2 regulatesCFTRexpression during endoderm differentiation and occupies 3′cis‐regulatory elements
| العنوان: | OTX2 regulatesCFTRexpression during endoderm differentiation and occupies 3′cis‐regulatory elements |
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| المؤلفون: | Shih Hsing Leir, Monali NandyMazumdar, Alekh Paranjapye, Shiyi Yin, Jenny L. Kerschner, Ann Harris |
| المصدر: | Developmental Dynamics. 250:684-700 |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Induced Pluripotent Stem Cells, Cystic Fibrosis Transmembrane Conductance Regulator, Respiratory Mucosa, 03 medical and health sciences, 0302 clinical medicine, Gene expression, medicine, Humans, Regulatory Elements, Transcriptional, Enhancer, Cells, Cultured, Regulation of gene expression, Otx Transcription Factors, biology, Endoderm, Cell Differentiation, respiratory system, Cystic fibrosis transmembrane conductance regulator, Cell biology, Chromatin, 030104 developmental biology, medicine.anatomical_structure, Histone, biology.protein, 030217 neurology & neurosurgery, Developmental Biology, Definitive endoderm |
| الوصف: | Background Cell-specific and developmental mechanisms contribute to expression of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, however, its developmental regulation is poorly understood. Here we use human induced pluripotent stem cells (hiPSCs) differentiated into pseudostratified airway epithelial cells to study these mechanisms. Results Changes in gene expression and open chromatin profiles were investigated by RNA-seq and ATAC-seq, and revealed that alterations in CFTR expression are associated with differences in stage-specific open chromatin. Additionally, 2 novel open chromatin regions, at +19.6kb and +22.6kb 3' to the CFTR translational stop signal, were observed in definitive endoderm (DE) cells, prior to an increase in CFTR expression in anterior foregut endoderm (AFE) cells. Chromatin studies in DE and AFE cells revealed enrichment of active enhancer marks and occupancy of OTX2 at these sites in DE cells. Loss of OTX2 in DE cells alters histone modifications across the CFTR locus and results in a 2.5-fold to 5-fold increase in CFTR expression. However, deletion of the +22.6kb site alone does not affect CFTR expression in DE or AFE cells. Conclusions These results suggest that a network of interacting cis-regulatory elements recruit OTX2 to the locus to impact CFTR expression during early endoderm differentiation. This article is protected by copyright. All rights reserved. |
| تدمد: | 1097-0177 1058-8388 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee50cbddfa62bb778a4fe4f646c28124 https://doi.org/10.1002/dvdy.293 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....ee50cbddfa62bb778a4fe4f646c28124 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970177 10588388 |
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