First identification and genotyping of Enterocytozoon bieneusi and Encephalitozoon spp. in pet rabbits in China
| العنوان: | First identification and genotyping of Enterocytozoon bieneusi and Encephalitozoon spp. in pet rabbits in China |
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| المؤلفون: | Ziyao Zhou, Lei Deng, Wuyou Wang, Yijun Chai, Guangneng Peng, Zhijun Zhong, Haifeng Liu, Hualin Fu, Leiqiong Xiang |
| المصدر: | BMC Veterinary Research BMC Veterinary Research, Vol 16, Iss 1, Pp 1-8 (2020) |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | China, Genotype, Microsporidiosis, Polymerase Chain Reaction, 030308 mycology & parasitology, 03 medical and health sciences, Feces, fluids and secretions, parasitic diseases, medicine, Prevalence, Animals, Enterocytozoon bieneusi, Encephalitozoon cuniculi, Genotyping, 030304 developmental biology, 0303 health sciences, lcsh:Veterinary medicine, General Veterinary, biology, fungi, virus diseases, Encephalitozoon, General Medicine, Enterocytozoon, biology.organism_classification, medicine.disease, Virology, Encephalitozoon intestinalis, Microsporidia, lcsh:SF600-1100, Rabbits, ITS, Research Article |
| الوصف: | Background Microsporidia are common opportunistic parasites in humans and animals, including rabbits. However, only limited epidemiology data concern about the prevalence and molecular characterization of Enterocytozoon bieneusi and Encephalitozoon spp. in rabbits. This study is the first detection and genotyping of Microsporidia in pet rabbits in China. Results A total of 584 faecal specimens were collected from rabbits in pet shops from four cities in Sichuan province, China. The overall prevalence of microsporidia infection was 24.8% by nested PCR targeting the internal transcribed spacer (ITS) region of E. bieneusi and Encephalitozoon spp. respectively. E. bieneusi was the most common species (n = 90, 15.4%), followed by Encephalitozoon cuniculi (n = 34, 5.8%) and Encephalitozoon intestinalis (n = 16, 2.7%). Mixed infections (E. bieneusi and E. cuniculi) were detected in five another rabbits (0.9%). Statistically significant differences in the prevalence of microsporidia were observed among different cities (χ2 = 38.376, df = 3, P 2 = 9.018, df = 2, P E. bieneusi were obtained, including five known (SC02, I, N, J, CHY1) and six novel genotypes (SCR01, SCR02, SCR04 to SCR07). SC02 was the most prevalent genotype in all tested cities (43.3%, 39/90). Phylogenetic analysis showed that these genotypes were clustered into group 1–3 and group 10. Meanwhile, two genotypes (I and II) were identified by sequence analysis of the ITS region of E. cuniculi. Conclusion To the best of our knowledge, this is the first report of microsporidia infection in pet rabbits in China. Genotype SC02 and four novel genotypes were classified into potential zoonotic group 1, suggesting that pet rabbits may cause microsporidiosis in humans through zoonotic transmissions. These findings provide preliminary reference data for monitoring microsporidia infections in pet rabbits and humans. |
| تدمد: | 1746-6148 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee54c170cbb83d773fc82d31dcece163 https://pubmed.ncbi.nlm.nih.gov/32571322 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ee54c170cbb83d773fc82d31dcece163 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 17466148 |
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