Tobamoviruses of two new species trigger resistance in pepper plants harbouring functional L alleles
| العنوان: | Tobamoviruses of two new species trigger resistance in pepper plants harbouring functional L alleles |
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| المؤلفون: | Tatsuya Nagata, Jefferson B. Vélez-Olmedo, Fernando L. Melo, Renato O. Resende, Athos Silva de Oliveira, C. E. Fribourg |
| المصدر: | Journal of General Virology. 102 |
| بيانات النشر: | Microbiology Society, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Pepper mild mottle virus, Genome, Viral, 01 natural sciences, Genome, Host Specificity, 03 medical and health sciences, Virology, Pepper, medicine, DNA Primers, Plant Diseases, Base Sequence, biology, Host (biology), Inoculation, Tobamovirus, fungi, food and beverages, biology.organism_classification, medicine.disease, Indicator plant, 030104 developmental biology, DNA, Viral, Mottle, Capsicum, 010606 plant biology & botany |
| الوصف: | Tobamoviruses are often referred to as the most notorious viral pathogens of pepper crops. These viruses are not transmitted by invertebrate vectors, but rather by physical contact and seeds. In this study, pepper plants displaying mild mottle and mosaic symptoms were sampled in four different regions of Peru. Upon double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) tests, seven samples cross-reacted weakly with antibodies against pepper mild mottle virus (PMMoV), suggesting the presence of tobamoviruses. When employing RT-PCR, conserved primers amplified cDNA fragments of viruses from two putative new tobamovirus species in the samples. The complete genome of two representative isolates were, therefore, sequenced and analysed in silico. These viruses, which were tentatively named yellow pepper mild mottle virus (YPMMoV) and chilli pepper mild mottle virus (CPMMoV), shared highest nucleotide genome sequence identities of 83 and 85 % with bell pepper mottle virus (BpeMV), respectively. Mechanical inoculation of indicator plants with YPMMoV and CPMMoV isolates did not show any obvious differences in host ranges. These viruses were also inoculated mechanically on pepper plants harbouring different resistance L alleles to determine their pathotypes. Pepper plants carrying unfunctional L alleles (L 0) to tobamoviruses were infected by all isolates and presented differential symptomatology for YPMMoV and CPMMoV. On the other hand, pepper plants carrying L 1, L 2, L 3 and L 4 alleles were resistant to all isolates, indicating that these viruses belong to pathotype P0. |
| تدمد: | 1465-2099 0022-1317 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee9c562d2827447c7065f63dfb958596 https://doi.org/10.1099/jgv.0.001524 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ee9c562d2827447c7065f63dfb958596 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14652099 00221317 |
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