High‐throughput seminal fluid identification by automated immunoaffinity mass spectrometry
| العنوان: | High‐throughput seminal fluid identification by automated immunoaffinity mass spectrometry |
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| المؤلفون: | Rachel, Davidovics, Yih Ling, Saw, Catherine O, Brown, Mechthild, Prinz, Heather E, McKiernan, Phillip B, Danielson, Kevin M, Legg |
| المصدر: | Journal of Forensic Sciences. 67:1184-1190 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Tandem Mass Spectrometry, Genetics, Humans, Proteins, Female, Peptides, Body Fluids, Chromatography, Liquid, Pathology and Forensic Medicine |
| الوصف: | The identification of semen during a criminal investigation may be a critical component in the prosecution of a sexual assault. Commonly employed enzymatic and affinity-based methods for detection lack specificity, are time-consuming, and only provide a presumptive indication that semen is present where microscopic visualization is unable to meet the throughput demands. Contrary to traditional approaches, protein mass spectrometry provides true confirmatory results, but multiday sample preparation and nanoflow sample separation requirements have limited the practical applicability of these approaches. Aiming at streamlining sexual assault screening by mass spectrometry, the work here coupled a 60-minute rapid tryptic digestion, semenogelin-II peptide affinity purification on an Agilent AssayMap Bravo automation platform, and a 3-minute targeted LC-MS/MS method on an Agilent 6495 triple quadrupole mass spectrometer operating in multiple reaction monitoring mode for detecting semenogelin-II peptides in sexual assault samples. The developed assay was assessed using casework-type samples and was successful in detecting trace levels (0.0001 μl) of semen recovered from both cotton and vaginal swabs, as well as semen recovered from vaginal swabs during menses or adulterated with personal lubricants. This work represents a promising technique for high-throughput seminal fluid identification in sexual assault-type samples by mass spectrometry. |
| تدمد: | 1556-4029 0022-1198 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eea896a19a980c61ee5906a2bdac1348 https://doi.org/10.1111/1556-4029.14975 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....eea896a19a980c61ee5906a2bdac1348 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15564029 00221198 |
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