Tracking T-cell immune reconstitution after TCRαβ/CD19-depleted hematopoietic cells transplantation in children
| العنوان: | Tracking T-cell immune reconstitution after TCRαβ/CD19-depleted hematopoietic cells transplantation in children |
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| المؤلفون: | Michael Maschan, A. A. Maschan, Sofya A. Kasatskaya, Ivan V. Zvyagin, Mikhail Shugay, Ekaterina A. Komech, D.N. Balashov, O V Tatarinova, Anastasiia L. Sycheva, V.V. Brilliantova, E V Boyakova, Larisa Shelikhova, Y B Lebedev, Elena Kurnikova, Dmitry M. Chudakov, Ilgar Z. Mamedov |
| المصدر: | Leukemia. 31:1145-1153 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Time Factors, Adolescent, Receptors, Antigen, T-Cell, alpha-beta, T-Lymphocytes, T cell, Antigens, CD19, Biology, Lymphocyte Depletion, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Immune system, Antigen, medicine, Humans, Progenitor cell, Child, Graft Survival, Hematopoietic Stem Cell Transplantation, Infant, Hematology, medicine.disease, Hematologic Diseases, 3. Good health, Transplantation, Leukemia, Haematopoiesis, 030104 developmental biology, medicine.anatomical_structure, Oncology, Child, Preschool, Immunology, Stem cell, 030215 immunology |
| الوصف: | αβT-cell-depleted allogeneic hematopoietic cell transplantation holds promise for the safe and accessible therapy of both malignant and non-malignant blood disorders. Here we employed molecular barcoding normalized T-cell receptor (TCR) profiling to quantitatively track T-cell immune reconstitution after TCRαβ-/CD19-depleted transplantation in children. We demonstrate that seemingly early reconstitution of αβT-cell counts 2 months after transplantation is based on only several hundred rapidly expanded clones originating from non-depleted graft cells. In further months, frequency of these hyperexpanded clones declines, and after 1 year the observed T-cell counts and TCRβ diversity are mostly provided by the newly produced T cells. We also demonstrate that high TCRβ diversity at day 60 observed for some of the patients is determined by recipient T cells and intrathymic progenitors that survived conditioning regimen. Our results indicate that further efforts on optimization of TCRαβ-/CD19-depleted transplantation protocols should be directed toward providing more efficient T-cell defense in the first months after transplantation. |
| تدمد: | 1476-5551 0887-6924 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eef0738cbf002eccf685c167abaa3f08 https://doi.org/10.1038/leu.2016.321 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....eef0738cbf002eccf685c167abaa3f08 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14765551 08876924 |
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