Simultaneous Qualitative and Quantitative Evaluation of the Coptidis Rhizoma and Euodiae Fructus Herbal Pair by Using UHPLC-ESI-QTOF-MS and UHPLC-DAD
| العنوان: | Simultaneous Qualitative and Quantitative Evaluation of the Coptidis Rhizoma and Euodiae Fructus Herbal Pair by Using UHPLC-ESI-QTOF-MS and UHPLC-DAD |
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| المؤلفون: | Min-Qun Guo, Xue-Mei Li, Xiu-Wei Yang, Yan-Ying Li |
| المصدر: | Molecules, Vol 25, Iss 4782, p 4782 (2020) Molecules Volume 25 Issue 20 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Esi qtof ms, Coptis chinensis, Spectrometry, Mass, Electrospray Ionization, Herbal Medicine, UHPLC-ESI-QTOF-MS, Pharmaceutical Science, Mass spectrometry, 01 natural sciences, Article, Gas Chromatography-Mass Spectrometry, Analytical Chemistry, Evodia, lcsh:QD241-441, 03 medical and health sciences, Qualitative analysis, Alkaloids, lcsh:Organic chemistry, Fingerprint, Drug Discovery, Humans, Physical and Theoretical Chemistry, Medicine, Chinese Traditional, Reference standards, Analysis method, Chromatography, High Pressure Liquid, 030304 developmental biology, Euodiae Fructus, Coptidis Rhizoma, 0303 health sciences, Chromatography, Chemistry, 010401 analytical chemistry, Organic Chemistry, Repeatability, 0104 chemical sciences, Chemistry (miscellaneous), Fruit, Molecular Medicine, quality markers, Ultra high performance, UHPLC-DAD fingerprint, herbal pair, Drugs, Chinese Herbal |
| الوصف: | The herbal pair of Coptidis Rhizoma (CR) and Euodiae Fructus (EF) is a classical traditional Chinese medicine formula used for treating gastro-intestinal disorders. In this study, we established a systematic method for chemical profiling and quantification analysis of the major constituents in the CR-EF herbal pair. A method of ultra high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) for qualitative analysis was developed. Sixty-five compounds, including alkaloids, phenolics, and limonoids, were identified or tentatively assigned by comparison with reference standards or literature data. The UHPLC fingerprints of 19 batches of the CR-EF herbal pair samples were obtained and the reference fingerprint chromatograms were established. Furthermore, nine compounds among 24 common peaks of fingerprints were considered as marker components, which either had high contents or significant bioactivities, were applied to quality control of the CR-EF herbal pair by quantitative analysis. This UHPLC-DAD analysis method was validated by precision, linearity, repeatability, stability, recovery, and so on. The method was simple and sensitive, and thus reliable for quantitative and chemical fingerprint analysis for the quality evaluation and control of the CR-EF herbal pair and related traditional Chinese medicines. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1420-3049 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef2ca1c3301da1dec2825c6ac5d1b20d https://www.mdpi.com/1420-3049/25/20/4782 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ef2ca1c3301da1dec2825c6ac5d1b20d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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