Comparative anatomy of the cerebellar cortex in mice lacking vimentin, GFAP, and both vimentin and GFAP
| العنوان: | Comparative anatomy of the cerebellar cortex in mice lacking vimentin, GFAP, and both vimentin and GFAP |
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| المؤلفون: | Véronique Menet, Francina Langa, Minerva Giménez y Ribotta, Alain Privat |
| المصدر: | Glia. 31:69-83 |
| بيانات النشر: | Wiley, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Mice, Knockout, Glia limitans, Cerebellum, Glial fibrillary acidic protein, biology, Vimentin, macromolecular substances, GFAP stain, Cell biology, Cerebellar Cortex, Mice, Microscopy, Electron, Cellular and Molecular Neuroscience, medicine.anatomical_structure, nervous system, Neurology, Cerebellar cortex, Glial Fibrillary Acidic Protein, medicine, biology.protein, Animals, Neuroglia, Neuron, Neuroscience |
| الوصف: | In the cerebellum of adult mammals, glial fibrillary acidic protein (GFAP) and vimentin (VIM) are coexpressed in Golgi epithelial cells (GEC), also known as Bergmann glia. In this study we used three transgenic knockout mice (GFAP, VIM and double GFAP and VIM) to analyze the involvement of these proteins in the building of glial filaments and in neuron-glia interactions. The cerebella of VIM, GFAP, and GFAP/VIM mutant mice were processed by the rapid Golgi method and also for electron microscopy. In VIM mutant mice, Bergmann fibers are hypertrophic with thickened appendages. In the electron microscope they appear as large glial profiles devoid of glial filaments, with embedded dendritic thorns and parallel fiber boutons. In addition, signs of degeneration are observed in Purkinje cells. In GFAP mutant mice, GEC exhibit fine, delicate processes, as those seen in wild-type animals, however, a large accumulation of lamellae and granular appendages was observed along their surfaces, which came into contact with each other. The electron microscope exhibited fine and scarce astroglial profiles containing some glial filaments, a stunted glia limitans, and the presence of large extracellular spaces. In double mutant mice, the two phenotypes are expressed but appear attenuated, with a total absence of glial filaments and the general appearance of immaturity for GEC. In conclusion, it appears that the absence of each of the proteins yields a specific phenotype and that the defects are not necessarily additive. |
| تدمد: | 1098-1136 0894-1491 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef3300a9098a4426c5f8c64fa09b05eb https://doi.org/10.1002/(sici)1098-1136(200007)31:1<69::aid-glia70>3.0.co;2-w |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....ef3300a9098a4426c5f8c64fa09b05eb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10981136 08941491 |
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