Expression levels of microRNA-199 and hypoxia-inducible factor-1 alpha in brain tissue of patients with intractable epilepsy
| العنوان: | Expression levels of microRNA-199 and hypoxia-inducible factor-1 alpha in brain tissue of patients with intractable epilepsy |
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| المؤلفون: | Xuzhi He, Xiaoming Wang, Ruijiao Zhou, Zhiqing Shi, Guohui Jiang, Min Huang, Juming Yu |
| المصدر: | International Journal of Neuroscience. 126:326-334 |
| بيانات النشر: | Informa UK Limited, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Drug Resistant Epilepsy, Pathology, medicine.medical_specialty, Adolescent, Angiogenesis, Gene Expression, Young Adult, 03 medical and health sciences, Hypoxia-Inducible Factor 1-Alpha, Epilepsy, 0302 clinical medicine, microRNA, medicine, Humans, Neocortex, business.industry, General Neuroscience, General Medicine, Middle Aged, Hypoxia (medical), Hypoxia-Inducible Factor 1, alpha Subunit, medicine.disease, Temporal Lobe, Pathophysiology, MicroRNAs, 030104 developmental biology, medicine.anatomical_structure, Case-Control Studies, Immunohistochemistry, Female, medicine.symptom, business, 030217 neurology & neurosurgery |
| الوصف: | During the last decade, experimental evidence has demonstrated an important role of hypoxia, which leads to neuronal cell death and angiogenesis, in the mechanisms of seizure precipitation and recurrence. MicroRNA-199 targets hypoxia-inducible factor-1alpha (HIF-1α), which has recently been implicated in the pathophysiology of the hypoxic state and brain injury. However, little is known about the roles of MicroRNA-199 and HIF-1α in the human epileptogenic process.In this study, we investigated the expression of miR-199a-5p, miR-199b-5p and HIF-1α using real-time PCR, immunohistochemistry and western blots in the temporal neocortex of twenty four patients with intractable epilepsy and twelve control subjects.Compared with the control group, the expression of miR-199a-5p and miR-199b-5p was significantly lower in epileptic brain tissues (p0.05). The levels of HIF-1α mRNA and protein were highly up-regulated in epileptic brain tissues compared with those of control subjects (p0.05).These data suggest that the abnormal expression of miR-199 and HIF-1α in epileptic brain tissue may be involved in the pathophysiology of human epilepsy and that the expression of HIF-1α may be regulated by miR-199. These findings may provide new insights into the treatment of epilepsy. |
| تدمد: | 1543-5245 0020-7454 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef528f55a7b41bea43e5cbe1788c356f https://doi.org/10.3109/00207454.2014.994209 |
| رقم الأكسشن: | edsair.doi.dedup.....ef528f55a7b41bea43e5cbe1788c356f |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 15435245 00207454 |
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