Features of anthocyanin biosynthesis in pap1-D and wild-type Arabidopsis thaliana plants grown in different light intensity and culture media conditions
| العنوان: | Features of anthocyanin biosynthesis in pap1-D and wild-type Arabidopsis thaliana plants grown in different light intensity and culture media conditions |
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| المؤلفون: | De-Yu Xie, Ming-Zhu Shi |
| المصدر: | Planta. 231:1385-1400 |
| بيانات النشر: | Springer Science and Business Media LLC, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Glycosylation, Light, Cyanidin, Mutant, Arabidopsis, Pancreatitis-Associated Proteins, Plant Science, Luminous intensity, Biology, Genes, Plant, Mass Spectrometry, Anthocyanins, chemistry.chemical_compound, Murashige and Skoog medium, Gene Expression Regulation, Plant, Botany, Genetics, RNA, Messenger, Chromatography, High Pressure Liquid, Genes, Dominant, Anthocyanidin, Arabidopsis Proteins, Pigmentation, fungi, Wild type, food and beverages, Biological Transport, Culture Media, Plant Leaves, Light intensity, chemistry, Biochemistry, Anthocyanin, Metabolic Networks and Pathways, Transcription Factors |
| الوصف: | The number of different anthocyanin molecules potentially produced by Arabidopsis thaliana and which anthocyanin molecule is the first product of anthocyanidin modification remain unknown. To accelerate the understanding of these questions, we investigated anthocyanin biosynthesis in rosette leaves of both pap1-D and wild-type (WT) A. thaliana plants grown in nine growth conditions, which were composed of three light intensities (low light, middle light, and high light) and three media derived from MS medium (medium-1, 2, and 3). These nine growth conditions differentially affected the levels of anthocyanins and pigmentation patterns of rosette leaves, which were closely related to the diversification levels of cyanin structures. The combined growth conditions of high light and either medium-2 or medium-1 induced the most molecular diversity of anthocyanin structures in rosette leaves of pap1-D plants. Twenty cyanin molecules, including five that were previously unknown, were characterized by HPLC-ESI-MS and HPLC-TOF-MS analyses. We detected that the A. thaliana anthocyanin molecule A11 was most likely the first cyanin derived from the multiple modification steps of cyanidin. In addition, in the same growth condition, rosette leaves of pap1-D plants produced much higher levels and more diverse molecular profiling of cyanins than those of WT plants. The transcript levels of PAP1, PAL1, CHS, DFR, and ANS cDNAs were much higher in pap1-D rosette leaves than in WT ones. Furthermore, on the same agar-solidified medium, an enhancement of light intensity increased levels and molecular diversity of cyanins in both pap1-D and WT rosette leaves. In the same light intensity condition, the responses of anthocyanin levels and profiling to medium alternation were different between pap1-D and WT plants. |
| تدمد: | 1432-2048 0032-0935 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef9bbc7b070721a4e65023c56f7afd45 https://doi.org/10.1007/s00425-010-1142-9 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....ef9bbc7b070721a4e65023c56f7afd45 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14322048 00320935 |
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