CILP2 overexpression correlates with tumor progression and poor prognosis in patients with colorectal cancer in The Cancer Genome Atlas (TCGA) study
| العنوان: | CILP2 overexpression correlates with tumor progression and poor prognosis in patients with colorectal cancer in The Cancer Genome Atlas (TCGA) study |
|---|---|
| المؤلفون: | Yangming Li, Yuanfei Peng, Feng Huang, Yangmei Xu, Lijie Huang, Qing Ye, Shengyuan Liu, Jinhu Chen |
| المصدر: | World Journal of Surgical Oncology World Journal of Surgical Oncology, Vol 18, Iss 1, Pp 1-10 (2020) |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Oncology, medicine.medical_specialty, Colorectal cancer, lcsh:Surgery, lcsh:RC254-282, 03 medical and health sciences, 0302 clinical medicine, Surgical oncology, Internal medicine, Biomarkers, Tumor, medicine, Humans, Survival analysis, CILP2, Tissue microarray, business.industry, Proportional hazards model, Research, lcsh:RD1-811, TCGA, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Prognosis, medicine.disease, Survival Analysis, Immunohistochemistry, 030104 developmental biology, Tumor progression, 030220 oncology & carcinogenesis, Colonic Neoplasms, Cohort, Surgery, Colorectal Neoplasms, business |
| الوصف: | Background Genetic alterations play an important role in the progression of colorectal cancer (CRC). Identifying new biomarkers to assess the prognosis of patients with CRC is critical. Cartilage intermediate layer protein 2 (CILP2) gene, screened from TCGA database by bioinformatics, may be closely related to the progression of CRC. CILP2 was barely reported with clinical features of tumors. Materials and methods Clinical information and RNA-seq data were derived from TCGA colorectal carcinoma cohort. CILP2 expression at mRNA level was estimated by bioinformatical analysis of TCGA cases. Tissue microarray (TMA) was constructed containing paraffin-embedded 64 pairs of CRC and matched adjacent normal tissues. The expression at the protein level was detected in 64 pairs of CRC and matched adjacent normal tissues by immunohistochemical analysis. CILP2 expression level and its clinical value were estimated by bioinformatical analysis with linear and logistic regression. Survival analysis was performed between high and low groups of CILP2 expression by Cox regression analysis, and the P value was calculated by the log-rank test. The Kaplan-Meier curves were tested by the log-rank test. Results CILP2 was statistically significantly higher expressed in the CRC tissues when compared with paired adjacent normal tissues in TCGA cohort (P < 0.001) and in the TMA cohort (P = 0.001). Also, CILP2 high expression was strongly correlated with T3/4 stage (P = 0.001), N1/2/3 stage (P = 0.005), M1 stage (P = 0.048), and higher clinical stage (UICC 2010 stage) (P < 0.001) in TCGA cohort, and also positively associated with T3/4 stage (P = 0.022) and higher clinical stage (UICC 2010 stage) (P = 0.03) in TMA cohort. Furthermore, CILP2 overexpression predicted poor prognosis and could be an independent prognostic factor (P = 0.003). Conclusion We revealed that CILP2 is associated with advanced stages and could play a role as an independent predictor of poor survival in CRC. |
| تدمد: | 1477-7819 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f141c06fec0fa1b1d8e3729185458b77 https://doi.org/10.1186/s12957-020-02049-6 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f141c06fec0fa1b1d8e3729185458b77 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14777819 |
|---|