التفاصيل البيبلوغرافية
| العنوان: |
Transformation of rat intestinal epithelial cells by overexpression of Rab25 is microtubule dependent |
| المؤلفون: |
James N. Higginbotham, Kenya M. Avant, Janine Hall, R. Daniel Beauchamp, Cathy M. Caldwell, Lynne A. Lapierre, James R. Goldenring |
| المصدر: |
Cytoskeleton. 68:97-111 |
| بيانات النشر: |
Wiley, 2011. |
| سنة النشر: |
2011 |
| مصطلحات موضوعية: |
Transplantation, Heterologous, Mice, Nude, Oncogene Protein p21(ras), Microtubules, Article, Microtubule polymerization, Focal adhesion, Mice, chemistry.chemical_compound, Intestinal mucosa, Structural Biology, Microtubule, Animals, Intestinal Mucosa, Cytoskeleton, Paxillin, biology, Epithelial Cells, Neoplasms, Experimental, Cell Biology, Rats, Cell biology, Gene Expression Regulation, Neoplastic, Nocodazole, Cell Transformation, Neoplastic, chemistry, rab GTP-Binding Proteins, Cancer cell, biology.protein, Cancer research, Neoplasm Transplantation |
| الوصف: |
Little research has addressed the role of membrane trafficking and recycling in the regulation of the transformed phenotype of neoplastic cells. The small GTPase Rab25 is an epithelial-specific modulator of membrane recycling. Recent studies have demonstrated that Rab25 expression is up-regulated in a number of epithelial cancers and overexpression may increase the aggressive phenotype of certain cancers. We have utilized the nontransformed RIE cell line to examine the influence of Rab25 on transformation. Overexpression of Rab25 in RIE cells leads to morphological transformation as well as growth in soft agar, tumor formation in nude mice, disruption of integrin-based focal adhesions, and alteration in modified microtubule subsets. Although the predominance of recent cancer research has focused on the manipulation of the actin-based cytoskeleton, recycling trafficking relies on microtubules. Transformation of RIE cells through overexpression of Rab25, but not with H-RasV12, was reversed by inhibitors of microtubule polymerization. These results suggest that up-regulation of Rab25 in RIE cells leads to microtubule-dependent transformation. Thus, depolymerization of microtubules may be a potent therapeutic target for cancer therapy through the reversal of the invasive phenotype of certain cancer cells. © 2011 Wiley-Liss, Inc. |
| تدمد: |
1949-3584 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f14f68c45c0e040f3013b42233eaa77a
https://doi.org/10.1002/cm.20497 |
| حقوق: |
OPEN |
| رقم الأكسشن: |
edsair.doi.dedup.....f14f68c45c0e040f3013b42233eaa77a |
| قاعدة البيانات: |
OpenAIRE |
