Characterizing human α-1,6-fucosyltransferase (FUT8) substrate specificity and structural similarities with related fucosyltransferases
| العنوان: | Characterizing human α-1,6-fucosyltransferase (FUT8) substrate specificity and structural similarities with related fucosyltransferases |
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| المؤلفون: | Boruah, Bhargavi M, Kadirvelraj, Renuka, Liu, Lin, Ramiah, Annapoorani, Li, Chao, Zong, Guanghui, Bosman, Gerlof P, Yang, Jeong-Yeh, Wang, Lai-Xi, Boons, Geert-Jan, Wood, Zachary A, Moremen, Kelley W, Afd Chemical Biology and Drug Discovery, Sub Chemical Biology and Drug Discovery, Chemical Biology and Drug Discovery |
| المساهمون: | Afd Chemical Biology and Drug Discovery, Sub Chemical Biology and Drug Discovery, Chemical Biology and Drug Discovery |
| المصدر: | J Biol Chem Journal of Biological Chemistry, 295(50), 17027. American Society for Biochemistry and Molecular Biology Inc. |
| بيانات النشر: | American Society for Biochemistry and Molecular Biology, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Glycan, Protein Folding, Fucosyltransferase, Glycobiology and Extracellular Matrices, Crystallography, X-Ray, Biochemistry, glycosyltransferase, Substrate Specificity, Fucosyltransferases, 03 medical and health sciences, N-linked glycosylation, Protein Domains, Humans, enzyme mechanism, Binding site, Molecular Biology, Fucosylation, 030102 biochemistry & molecular biology, biology, Glycobiology, Chemistry, substrate recognition, Active site, Cell Biology, 030104 developmental biology, HEK293 Cells, Structural Homology, Protein, biology.protein |
| الوصف: | Mammalian Asn-linked glycans are extensively processed as they transit the secretory pathway to generate diverse glycans on cell surface and secreted glycoproteins. Additional modification of the glycan core by α-1,6-fucose addition to the innermost GlcNAc residue (core fucosylation) is catalyzed by an α-1,6-fucosyltransferase (FUT8). The importance of core fucosylation can be seen in the complex pathological phenotypes of FUT8 null mice, which display defects in cellular signaling, development, and subsequent neonatal lethality. Elevated core fucosylation has also been identified in several human cancers. However, the structural basis for FUT8 substrate specificity remains unknown. Here, using various crystal structures of FUT8 in complex with a donor substrate analog, and with four distinct glycan acceptors, we identify the molecular basis for FUT8 specificity and activity. The ordering of three active site loops corresponds to an increased occupancy for bound GDP, suggesting an induced-fit folding of the donor-binding subsite. Structures of the various acceptor complexes were compared with kinetic data on FUT8 active site mutants and with specificity data from a library of glycan acceptors to reveal how binding site complementarity and steric hindrance can tune substrate affinity. The FUT8 structure was also compared with other known fucosyltransferases to identify conserved and divergent structural features for donor and acceptor recognition and catalysis. These data provide insights into the evolution of modular templates for donor and acceptor recognition among GT-B fold glycosyltransferases in the synthesis of diverse glycan structures in biological systems. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0021-9258 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f151200fce067240cd757c0e35681dd1 https://europepmc.org/articles/PMC7863877/ |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f151200fce067240cd757c0e35681dd1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219258 |
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