The mRNA Binding Proteome of Proliferating and Differentiated Muscle Cells
| العنوان: | The mRNA Binding Proteome of Proliferating and Differentiated Muscle Cells |
|---|---|
| المؤلفون: | Annemieke Aartsma-Rus, Monika Hiller, Miriam Geissler, George M.C. Janssen, Pietro Spitali, Peter A. van Veelen |
| المصدر: | GENOMICS PROTEOMICS & BIOINFORMATICS, 18(4), 384-396. ELSEVIER Genomics, Proteomics & Bioinformatics |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Duchenne muscular dystrophy, Proteomics, Interactome, Proteome, Skeletal muscle, RNA-binding protein, Biology, Biochemistry, Transcriptome, 03 medical and health sciences, 0302 clinical medicine, Gene expression, Genetics, medicine, Humans, Myocyte, Myocytes, Cardiac, RNA, Messenger, Molecular Biology, Gene, Original Research, 030304 developmental biology, 0303 health sciences, Myogenesis, Gene Expression Profiling, RNA-Binding Proteins, RNA, RNA binding protein, Cell biology, Computational Mathematics, medicine.anatomical_structure, 030217 neurology & neurosurgery |
| الوصف: | Muscle formation is a coordinated process driven by extensive gene expression changes where single cells fuse together to form multinucleated muscle fibers. Newly synthesized mRNAs are then regulated by RNA binding proteins (RBPs), affecting post-transcriptional transcript metabolism. Here, we determined how large-scale gene expression changes affect the catalog of RBPs by studying proliferating and differentiated muscle cells in healthy and dystrophic conditions. Transcriptomic analysis showed that the expression of more than 7000 genes was affected during myogenesis. We identified 769 RBPs, of which 294 were muscle-specific and 49 were uniquely shared with cardiomyocytes. A subset of 32 RBPs (half of which were muscle-specific) was found to be preferentially associated with target mRNAs in either myoblasts (MBs) or myotubes (MTs). A large proportion of catalytic proteins were bound to mRNAs even though they lack classical RNA binding domains. Finally, we showed how the identification of cell-specific RBPs enabled the identification of biomarkers that can separate healthy individuals from dystrophic patients. Our data show how interactome data can shed light on new basic RNA biology as well as provide cell-specific data that can be used for diagnostic purposes. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f18f51c31233ad92bc07dd49e85cec44 https://hdl.handle.net/1887/3212835 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f18f51c31233ad92bc07dd49e85cec44 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |