The Ralstonia solanacearum effector RipI induces a defence reaction by interacting with the bHLH93 transcription factor in Nicotiana benthamiana
| العنوان: | The Ralstonia solanacearum effector RipI induces a defence reaction by interacting with the bHLH93 transcription factor in Nicotiana benthamiana |
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| المؤلفون: | Tao Zhuo, Xun Hu, Yanhong Zeng, Zhengyu Chen, Haitao Cui, Xiaojing Fan, Yang Chen, Xue Wang, Huasong Zou |
| المصدر: | Molecular Plant Pathology |
| بيانات النشر: | John Wiley and Sons Inc., 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Hypersensitive response, RipI, Short Communication, Soil Science, Nicotiana benthamiana, Virulence, defence reaction, Plant Science, 01 natural sciences, 03 medical and health sciences, Bacterial Proteins, Gene Expression Regulation, Plant, Arabidopsis, Tobacco, Basic Helix-Loop-Helix Transcription Factors, Gene silencing, Molecular Biology, Transcription factor, transcription factor, Plant Diseases, Plant Proteins, Ralstonia solanacearum, biology, Effector, fungi, food and beverages, bHLH93, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Cell biology, Up-Regulation, 030104 developmental biology, Agronomy and Crop Science, 010606 plant biology & botany |
| الوصف: | Ralstonia solanacearum releases a set of effectors into plant cells that modify the host defence reaction. The role of the effector protein RipI during infection has not been elucidated. In this study, we demonstrated that transient overexpression of RipI induces the hypersensitive response (HR), up‐regulating the HR marker gene hin1, in Nicotiana benthamiana. Deletion of R. solanacearum ripI led to increased virulence in tomato (Solanum lycopersicum) plants. Through yeast two‐hybrid and pull‐down assays, we identified an interaction between the N. benthamiana transcription factor bHLH93 and RipI, both of which could be localized in the nucleus of Arabidopsis protoplasts. Silencing of bHLH93 markedly attenuated the RipI‐induced HR and induced expression of the PDF1.2 defence gene. These data demonstrate that the R. solanacearum effector RipI induces a host defence reaction by interacting with the bHLH93 transcription factor. Ralstonia solanacearum RipI is recognized by bHLH93 to induce a defence reaction in tobacco plants. |
| اللغة: | English |
| تدمد: | 1364-3703 1464-6722 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1c1a6e153b1a3ceb6845654c11e55a2 http://europepmc.org/articles/PMC7279998 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f1c1a6e153b1a3ceb6845654c11e55a2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13643703 14646722 |
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