Costunolide induces apoptosis and inhibits migration and invasion in H1299 lung cancer cells
| العنوان: | Costunolide induces apoptosis and inhibits migration and invasion in H1299 lung cancer cells |
|---|---|
| المؤلفون: | Yan-Rui Pan, Xiao-Yun Shen, Chaoyue Su, Peiquan Zhang, Qiao-Ru Guo, Jianye Zhang, Jia-Jun Li, Minyan Wei, Zengbao Wu, Hui Wang, Yun Liu, Jianhua Qiu, Yanyan Yan |
| المصدر: | Oncology Reports |
| بيانات النشر: | D.A. Spandidos, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Epithelial-Mesenchymal Transition, Lung Neoplasms, Cell Survival, Cell, anticancer, migration, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Annexin, Cell Movement, Carcinoma, Non-Small-Cell Lung, Cell Line, Tumor, medicine, Humans, MTT assay, Gene Regulatory Networks, Viability assay, Propidium iodide, costunolide, Cell Proliferation, Costunolide, General Medicine, Articles, Cell cycle, invasion, Antineoplastic Agents, Phytogenic, Gene Expression Regulation, Neoplastic, 030104 developmental biology, medicine.anatomical_structure, Oncology, chemistry, non-small-cell lung cancer, Apoptosis, 030220 oncology & carcinogenesis, Cancer research, epithelial-to-mesenchymal transition, Sesquiterpenes, Signal Transduction |
| الوصف: | Costunolide being a sesquiterpene lactone, is known to have anticancer properties. The present study investigated the anticancer effects of costunolide against the H1299 human non‑small‑cell lung cancer (NSCLC) cell line. Inhibition of cell viability by costunolide was assessed via a MTT assay. Furthermore, the apoptotic rate was detected using Annexin V/propidium iodide labeling. A colony forming cell assay was performed to investigate the antiproliferative effects of costunolide. Wound healing and Transwell assays were performed to determine the inhibitory effects of costunolide on migration and invasion, respectively. Western blot analysis was undertaken to determine protein expression, and reverse transcription‑quantitative PCR was performed to assess mRNA expression levels. The results demonstrated that costunolide inhibited the viability of H1299 cells, with a half maximal inhibitory concentration value of 23.93±1.67 µM and induced cellular apoptosis in a dose‑dependent manner. Furthermore, the colony formation, migrative and invasive abilities of the H1299 cells were inhibited in a dose‑ or time‑dependent manner. The protein expression levels of E‑cadherin increased and those of N‑cadherin decreased following treatment with costunolide, which suggested that costunolide inhibited epithelial‑to‑mesenchymal transition. The mRNA levels of B‑Raf, E‑cadherin, N‑cadherin, integrins α2 and β1, as well as matrix metalloproteinases 2 were also found to be regulated costunolide. These findings indicate the potential of costunolide in the treatment of NSCLC. |
| اللغة: | English |
| تدمد: | 1791-2431 1021-335X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f23363fc2a9d75f2351a8877d12db4b6 http://europepmc.org/articles/PMC7160540 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f23363fc2a9d75f2351a8877d12db4b6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912431 1021335X |
|---|