Deciphering the regulatory genome of Escherichia coli, one hundred promoters at a time
| العنوان: | Deciphering the regulatory genome of Escherichia coli, one hundred promoters at a time |
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| المؤلفون: | Nicholas S. McCarty, Emanuel Flores-Bautista, Justin B. Kinney, Tom Röschinger, Suzannah M. Beeler, William T. Ireland, Nathan M. Belliveau, Annie Moradian, Rob Phillips, Michael J. Sweredoski |
| المصدر: | eLife eLife, Vol 9 (2020) |
| بيانات النشر: | eLife Sciences Publications, Ltd, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Base pair, QH301-705.5, Science, ved/biology.organism_classification_rank.species, Computational biology, Biology, medicine.disease_cause, Physics of Living Systems, Genome, General Biochemistry, Genetics and Molecular Biology, DNA sequencing, 03 medical and health sciences, 0302 clinical medicine, transcription factors, Transcriptional regulation, medicine, Escherichia coli, transcriptional regulation, Biology (General), Model organism, Promoter Regions, Genetic, Transcription factor, 030304 developmental biology, information theory, 0303 health sciences, General Immunology and Microbiology, ved/biology, General Neuroscience, E. coli, Promoter, quantitative biology, General Medicine, Gene Expression Regulation, Bacterial, Sequence Analysis, DNA, gene expression, Medicine, 030217 neurology & neurosurgery, Genome, Bacterial, Research Article |
| الوصف: | Advances in DNA sequencing have revolutionized our ability to read genomes. However, even in the most well-studied of organisms, the bacteriumEscherichia coli, for ≈65% of promoters we remain ignorant of their regulation. Until we crack this regulatory Rosetta Stone, efforts to read and write genomes will remain haphazard. We introduce a new method, Reg-Seq, that links massively parallel reporter assays with mass spectrometry to produce a base pair resolution dissection of more than aE. colipromoters in 12 growth conditions. We demonstrate that the method recapitulates known regulatory information. Then, we examine regulatory architectures for more than 80 promoters which previously had no known regulatory information. In many cases, we also identify which transcription factors mediate their regulation. This method clears a path for highly multiplexed investigations of the regulatory genome of model organisms, with the potential of moving to an array of microbes of ecological and medical relevance. |
| وصف الملف: | application/pdf; application/vnd.ms-excel; application/x-gzip; application/msword; text/plain |
| اللغة: | English |
| تدمد: | 2050-084X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2c073c294343ac9f5a3f69c97732ce4 http://europepmc.org/articles/PMC7567609 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f2c073c294343ac9f5a3f69c97732ce4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2050084X |
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