Muscle Protein Signaling in C2C12 Cells Is Stimulated to Similar Degrees by Diverse Commercial Food Protein Sources and Experimental Soy Protein Hydrolysates
| العنوان: | Muscle Protein Signaling in C2C12 Cells Is Stimulated to Similar Degrees by Diverse Commercial Food Protein Sources and Experimental Soy Protein Hydrolysates |
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| المؤلفون: | Naina K. Shah, Dustie N Butteiger, Elaine S. Krul, David A Roeseler, Janine Hall-Porter, Nancy J McGraw, Ratna Mukherjea |
| المصدر: | Journal of Agricultural and Food Chemistry. 65:2956-2964 |
| بيانات النشر: | American Chemical Society (ACS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, General Chemistry, mTORC1, Biology, Hydrolysate, 03 medical and health sciences, 030104 developmental biology, Biochemistry, biology.protein, Phosphorylation, Food science, Signal transduction, General Agricultural and Biological Sciences, Digestion, Soy protein, Mechanistic target of rapamycin, C2C12 |
| الوصف: | Dietary protein stimulates muscle protein synthesis and is essential for muscle health. We developed a screening assay using C2C12 mouse muscle cells to assess the relative abilities of diverse commercial protein sources and experimental soy protein hydrolysates (ESH), after simulated gut digestion (SGD), to activate the mechanistic target of rapamycin complex I (mTORC1) muscle protein synthesis signaling pathway (p70S6K(Thr389) phosphorylation). Activation of mTORC1 was expressed as a percentage of a maximal insulin response. The bioactivities of proteins grouped by source including fish (81.3 ± 10.6%), soy (66.2 ± 4.7%), dairy (61.8 ± 4.3%), beef (53.7 ± 8.6%), egg (52.3 ± 10.6%), soy whey (43.4 ± 8.6%), and pea (31.4 ± 10.6%) were not significantly different from each other. Bioactivity for ESH ranged from 28.0 ± 7.5 to 98.2 ± 6.6%. The results indicate that both the protein source and processing conditions are key determinants for mTORC1 activation. Regression analyses demonstrated that neither leucin... |
| تدمد: | 1520-5118 0021-8561 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2dd25c4f22028c8a08c21515a12a738 https://doi.org/10.1021/acs.jafc.6b05460 |
| رقم الأكسشن: | edsair.doi.dedup.....f2dd25c4f22028c8a08c21515a12a738 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15205118 00218561 |
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