Analytical performance specifications for the measurement uncertainty of 24,25-dihydroxyvitamin D examinations
| العنوان: | Analytical performance specifications for the measurement uncertainty of 24,25-dihydroxyvitamin D examinations |
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| المؤلفون: | Etienne Cavalier, Callum G. Fraser, Harjit Pal Bhattoa, Annemieke C. Heijboer, Konstantinos Makris, Samuel Vasikaran, Loreen Huyghebaert, Stéphanie Peeters, Caroline Le Goff, Markus Herrmann, Anna Carobene |
| المساهمون: | Endocrinology Laboratory, Amsterdam Reproduction & Development (AR&D), Amsterdam Gastroenterology Endocrinology Metabolism |
| المصدر: | Clinical Chemistry and Laboratory Medicine. Walter de Gruyter GmbH IFCC-IOF Committee for Bone Metabolism 2023, ' Analytical performance specifications for the measurement uncertainty of 24,25-dihydroxyvitamin D examinations ', Clinical Chemistry and Laboratory Medicine . https://doi.org/10.1515/cclm-2023-0176 |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Biochemistry (medical), Clinical Biochemistry, General Medicine |
| الوصف: | Objectives The exploration of the metabolites in the degradation pathways of vitamin D (VTD) has gained importance in recent years and simultaneous quantitation of twenty-five-hydroxy vitamin D (25(OH)D) mass concentration together with 24,25-dihydroxyvitamin D (24,25(OH)2D) has been proposed as a newer approach to define VTD deficiency. Yet, no data are available on 24,25(OH)2D biological variation (BV). In this study, we evaluated 24,25(OH)2D’s BV on the European Biological Variation Study (EuBIVAS) cohort samples to determine if analytical performance specifications (APS) for 24,25(OH)2D could be generated. Methods Six European laboratories recruited 91 healthy participants. 25(OH)D and 24,25(OH)2D concentrations in K3-EDTA plasma were examined weekly for up to 10 weeks in duplicate with a validated LC-MS/MS method. The Vitamin D Metabolite Ratio (24,25(OH)2D divided by 25(OH)D × 100) was also calculated at each time point. Results Linear regression of the mean 24,25(OH)2D concentrations at each blood collection showed participants were not in steady state. Variations of 24,25(OH)2D over time were significantly positively associated with the slopes of 25(OH)D concentrations over time and the concentration of 25(OH)D of the participant at inclusion, and negatively associated with body mass index (BMI), but not with age, gender, or location of the participant. The variation of the 24,25(OH)2D concentration in participants over a 10 weeks period was 34.6%. Methods that would detect a significant change linked to the natural production of 24,25(OH)2D over this period at pu%) Conclusions We have defined for the first time APS for 24,25(OH)2D examinations. According to the growing interest in this metabolite, several laboratories and manufacturers might aim to develop specific methods for its determination. The results presented in this paper are thus necessary prerequisites for the validation of such methods. |
| اللغة: | English |
| تدمد: | 1434-6621 2023-0176 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f36e2a86fc00dfcdccd5b0a646f4ba02 http://www.scopus.com/inward/record.url?scp=85151876783&partnerID=8YFLogxK |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f36e2a86fc00dfcdccd5b0a646f4ba02 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14346621 20230176 |
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