Dynamic hydrolase activities precede hypersensitive tissue collapse in tomato seedlings
| العنوان: | Dynamic hydrolase activities precede hypersensitive tissue collapse in tomato seedlings |
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| المؤلفون: | Matthieu H. A. J. Joosten, Renier A. L. van der Hoorn, Daniela J. Sueldo, Wladimir I. L. Tameling, Johana C. Misas-Villamil, Tom Colby, Ali Abdurehim Ahmed |
| المصدر: | New Phytologist, 203(3), 913-925 New Phytologist New Phytologist 203 (2014) 3 |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Proteases, Programmed cell death, disease resistance, Physiology, Intracellular Space, Plant Science, Biology, Subtilase, cysteine proteases, Serine, Solanum lycopersicum, Gene expression, Hydrolase, pathogenesis-related proteins, programmed cell-death, chemistry.chemical_classification, EPS-2, vacuolar processing enzyme, fungi, Temperature, food and beverages, Humidity, gene-expression, Laboratorium voor Phytopathologie, arabidopsis, Enzyme, Proteasome, Biochemistry, chemistry, Seedlings, plant-pathogen, Laboratory of Phytopathology, defense responses, activation, Serine Proteases, Extracellular Space, Cladosporium |
| الوصف: | Summary Hydrolases such as subtilases, vacuolar processing enzymes (VPEs) and the proteasome play important roles during plant programmed cell death (PCD). We investigated hydrolase activities during PCD using activity-based protein profiling (ABPP), which displays the active proteome using probes that react covalently with the active site of proteins. We employed tomato (Solanum lycopersicum) seedlings undergoing synchronized hypersensitive cell death by co-expressing the avirulence protein Avr4 from Cladosporium fulvum and the tomato resistance protein Cf-4. Cell death is blocked in seedlings grown at high temperature and humidity, and is synchronously induced by decreasing temperature and humidity. ABPP revealed that VPEs and the proteasome are not differentially active, but that activities of papain-like cysteine proteases and serine hydrolases, including Hsr203 and P69B, increase before hypersensitive tissue collapse, whereas the activity of a carboxypeptidase-like enzyme is reduced. Similar dynamics were observed for these enzymes in the apoplast of tomato challenged with C. fulvum. Unexpectedly, these challenged plants also displayed novel isoforms of secreted putative VPEs. In the absence of tissue collapse at high humidity, the hydrolase activity profile is already altered completely, demonstrating that changes in hydrolase activities precede hypersensitive tissue collapse. |
| وصف الملف: | application/octet-stream; application/pdf |
| تدمد: | 1469-8137 0028-646X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3aff1880ecf69cd21509edf97f0683c https://pubmed.ncbi.nlm.nih.gov/24890496 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f3aff1880ecf69cd21509edf97f0683c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14698137 0028646X |
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