Infection of porcine bone marrow-derived macrophages by porcine respiratory and reproductive syndrome virus impairs phagosomal maturation
| العنوان: | Infection of porcine bone marrow-derived macrophages by porcine respiratory and reproductive syndrome virus impairs phagosomal maturation |
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| المؤلفون: | Robin M. Yates, Neil McKenna, Dale R. Balce, Sibapriya Chaudhuri |
| المصدر: | Journal of General Virology. 97:669-679 |
| بيانات النشر: | Microbiology Society, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Phagocyte, Swine, 040301 veterinary sciences, Phagocytosis, Population, Porcine Reproductive and Respiratory Syndrome, Bone Marrow Cells, Biology, Virus, 0403 veterinary science, 03 medical and health sciences, Phagosomes, Virology, medicine, Animals, Macrophage, Porcine respiratory and reproductive syndrome virus, education, Cells, Cultured, Phagosome, education.field_of_study, Lung, Macrophages, 04 agricultural and veterinary sciences, Porcine reproductive and respiratory syndrome virus, biology.organism_classification, 030104 developmental biology, medicine.anatomical_structure |
| الوصف: | Porcine reproductive and respiratory syndrome virus (PRRSV), a positive-sense, ssRNA virus of the genus Arterivirus, is a devastating disease of swine worldwide. Key early targets of PRRSV infection in pigs include professional phagocytes in the lung, such as alveolar and interstitial macrophages and dendritic cells, the dysfunction of which is believed to be responsible for much of the associated mortality. In order to study the effect of virus infection on phagocyte function, the development of a robust, reproducible model would be advantageous. Given the limitations of current models, we set out to develop a porcine bone marrow-derived macrophage (PBMMΦ) cell model to study phagosomal maturation and function during PRRSV infection. Derivation of PBMMΦs from marrow using cultured L929 fibroblast supernatant produced a homogeneous population of cells that exhibited macrophage-like morphology and proficiency in Fc-receptor-mediated phagocytosis and phagosomal maturation. PBMMΦs were permissive to PRRSV infection, resulting in a productive infection that peaked at 24 h. Assessment of the effect of PRRSV infection on the properties of phagosomal maturation in PBMMΦs revealed a significant decrease in phagosomal proteolysis and lowered production of reactive oxygen species, but no change in PBMMΦ viability, phagocytosis or the ability of phagosomes to acidify. In this study, we present a new model to investigate PRRSV infection of phagocytes, which demonstrates a significant effect on phagosomal maturation with the associated implications on proper macrophage function. This model can also be used to study the effect on the phagosomal microenvironment of infection by other viruses targeting porcine macrophages. |
| تدمد: | 1465-2099 0022-1317 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3b2df68efed06c051f84873df16bb6a https://doi.org/10.1099/jgv.0.000384 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f3b2df68efed06c051f84873df16bb6a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14652099 00221317 |
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