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Rapid efficient synthesis and characterization of silver, gold, and bimetallic nanoparticles from the medicinal plant Plumbago zeylanica and their application in biofilm control

التفاصيل البيبلوغرافية
العنوان: Rapid efficient synthesis and characterization of silver, gold, and bimetallic nanoparticles from the medicinal plant Plumbago zeylanica and their application in biofilm control
المؤلفون: Balu A. Chopade, Trupti Kale, Jayesh R. Bellare, Gopal C. Kundu, Vikas Pruthi, Gayatri R Salunke, Samiksha Khade, Priya Vashisth, Snehal Chopade, R. J. Santosh Kumar, Sougata Ghosh
المصدر: International Journal of Nanomedicine
International Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2635-2653 (2014)
بيانات النشر: Dove Press, 2014.
سنة النشر: 2014
مصطلحات موضوعية: Plumbago zeylanica, Biofilm Inhibition And Disruption, Medicine (General), Materials science, Biophysics, Pharmaceutical Science, Nanoparticle, Metal Nanoparticles, Bioengineering, Apoptosis, P. Zeylanica, Aunps, Plant Roots, Microbiology, Biomaterials, chemistry.chemical_compound, R5-920, Plumbaginaceae, Dynamic light scattering, International Journal of Nanomedicine, Drug Discovery, Agaunps, Alloys, Original Research, Aqueous solution, Plants, Medicinal, biology, Plant Extracts, Organic Chemistry, Biofilm, General Medicine, biology.organism_classification, Gc-Tof-Ms, Anti-Bacterial Agents, Agnps, Silver nitrate, chemistry, Biofilms, Chloroauric acid, Citric acid, Nuclear chemistry
الوصف: Gayatri R Salunke,1 Sougata Ghosh,1 RJ Santosh Kumar,2 Samiksha Khade,1 Priya Vashisth,3 Trupti Kale,4 Snehal Chopade,5 Vikas Pruthi,3 Gopal Kundu,4 Jayesh R Bellare,6 Balu A Chopade1,5 1Institute of Bioinformatics and Biotechnology, University of Pune, 2National Chemical Laboratory, Pune, 3Department of Biotechnology, Indian Institute of Technology, Roorkee, 4National Centre for Cell Science, Pune University Complex, 5Department of Microbiology, University of Pune, Pune, 6Department of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, India Background: Nanoparticles (NPs) have gained significance in medical fields due to their high surface-area-to-volume ratio. In this study, we synthesized NPs from a medicinally important plant – Plumbago zeylanica. Materials and methods: Aqueous root extract of P. zeylanica (PZRE) was analyzed for the presence of flavonoids, sugars, and organic acids using high-performance thin-layer chromatography (HPTLC), gas chromatography-time of flight-mass spectrometry (GC-TOF-MS), and biochemical methods. The silver NPs (AgNPs), gold NPs (AuNPs), and bimetallic NPs (AgAuNPs) were synthesized from root extract and characterized using ultraviolet-visible spectra, X-ray diffraction (XRD), energy-dispersive spectrometry (EDS), transmission electron microscopy (TEM), and dynamic light scattering (DLS). The effects of these NPs on Acinetobacter baumannii, Staphylococcus aureus, and Escherichia coli biofilms were studied using quantitative biofilm inhibition and disruption assays, as well as using fluorescence, ­scanning electron microscopy, and atomic force microscopy. Results: PZRE showed the presence of phenolics, such as plumbagin, and flavonoids, in addition to citric acid, sucrose, glucose, fructose, and starch, using HPTLC, GC-TOF-MS, and quantitative analysis. Bioreduction of silver nitrate (AgNO3) and chloroauric acid (HAuCl4) were confirmed at absorbances of440nm (AgNPs),570nm (AuNPs), and540nm (AgAuNPs), respectively. The maximum rate of synthesis at 50°C was achieved with 5 mM AgNO3 within 4.5 hours for AgNPs; and with 0.7 mM HAuCl4 within 5 hours for AuNPs. The synthesis of AgAuNPs, which completed within 90 minutes with 0.7 mM AgNO3 and HAuCl4, was found to be the fastest. Fourier-transform infrared spectroscopy confirmed bioreduction, while EDS and XRD patterns confirmed purity and the crystalline nature of the NPs, respectively. TEM micrographs and DLS showed about60nm monodispersed Ag nanospheres,20–30nm Au nanospheres adhering to form Au nanotriangles, and about90nm hexagonal blunt-ended AgAuNPs. These NPs also showed antimicrobial and antibiofilm activity against E. coli, A. baumannii, S. aureus, and a mixed culture of A. baumannii and S. aureus. AgNPs inhibited biofilm in the range of96%–99% and AgAuNPs from93% to98% in single-culture biofilms. AuNPs also showed biofilm inhibition, with the highest of98% in S. aureus. AgNPs also showed good biofilm disruption, with the highest of88% in A. baumannii. Conclusion: This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms. Keywords: P. zeylanica, AgNPs, AuNPs, AgAuNPs, biofilm inhibition and disruption, GC-TOF-MS
وصف الملف: text/html
اللغة: English
تدمد: 1178-2013
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4173bb092d8e9c0879f2ab60d4a0aba
https://www.dovepress.com/rapid-efficient-synthesis-and-characterization-of-silver-gold-and-bime-peer-reviewed-article-IJN
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....f4173bb092d8e9c0879f2ab60d4a0aba
قاعدة البيانات: OpenAIRE
الوصف
تدمد:11782013