Measurements of 3-D morphology of individual red blood cells upon localized photothermal effects using gold nanorods

التفاصيل البيبلوغرافية
العنوان: Measurements of 3-D morphology of individual red blood cells upon localized photothermal effects using gold nanorods
المؤلفون: Juntaek Oh, Chanwoo Song, Yoonkey Nam, Gu-Haeng Lee, YongKeun Park, SangYun Lee, Seungwoo Shin, Moosung Lee
بيانات النشر: Cold Spring Harbor Laboratory, 2019.
سنة النشر: 2019
مصطلحات موضوعية: Materials science, Heat generation, Excited state, Near-infrared spectroscopy, Biophysics, Nanorod, Photothermal therapy, Cell morphology, Refractive index, Excitation
الوصف: We investigated the in-situ photothermal response of human red blood cells (RBCs) by combining photothermal heat generation and 3D quantitative phase imaging techniques. Gold-nanorod-coated substrates were excited using near-infrared light to generate local heat to RBCs, and response was measured by imaging 3-D refractive index tomograms of cells under various near infrared (NIR) excitation conditions. On photothermal treatment, cell morphology changed from discoid to crescent shapes, cell volume and dry mass decreased, and hemoglobin concentration increased. We also investigated the irreversible deformation of RBCs when multiple intense excitation shocks are applied. These results provide a new understanding of thermodynamic aspects of cell biology and hematology. O_FIG_DISPLAY_L [Figure 1] M_FIG_DISPLAY C_FIG_DISPLAY
اللغة: English
DOI: 10.1101/2019.12.11.872218
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4c2aa503dff6c648bbb089109b999b6
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....f4c2aa503dff6c648bbb089109b999b6
قاعدة البيانات: OpenAIRE
الوصف
DOI:10.1101/2019.12.11.872218