Respiratory syncytial virus infection of human respiratory epithelial cells enhances inducible nitric oxide synthase gene expression
| العنوان: | Respiratory syncytial virus infection of human respiratory epithelial cells enhances inducible nitric oxide synthase gene expression |
|---|---|
| المؤلفون: | Masaya Ohsaki, Shunzo Chiba, Kimihira Seki, Ryoh Takeuchi, Hiroyuki Tsutsumi |
| المصدر: | Journal of Leukocyte Biology. 66:99-104 |
| بيانات النشر: | Oxford University Press (OUP), 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | medicine.medical_treatment, Blotting, Western, Immunology, Nitric Oxide Synthase Type II, Respiratory Syncytial Virus Infections, Biology, Gene Expression Regulation, Enzymologic, Proinflammatory cytokine, Nitric oxide, Microbiology, Interferon-gamma, chemistry.chemical_compound, Neutralization Tests, Nasopharynx, Gene expression, Tumor Cells, Cultured, medicine, Humans, Immunology and Allergy, Nitrites, A549 cell, Regulation of gene expression, Tumor Necrosis Factor-alpha, Infant, Interferon-alpha, Epithelial Cells, Exudates and Transudates, Interferon-beta, Cell Biology, Phosphoproteins, DNA-Binding Proteins, Pulmonary Alveoli, Nitric oxide synthase, IRF1, Cytokine, Gene Expression Regulation, chemistry, Enzyme Induction, Respiratory Syncytial Virus, Human, biology.protein, Nitric Oxide Synthase, Interferon Regulatory Factor-1, Interleukin-1 |
| الوصف: | The induction kinetics of the mRNA of interferon regulatory factor 1 (IRF-1), inducible nitric oxide synthase (iNOS), and proinflammatory cytokines in respiratory syncytial virus (RSV)-infected human type 2 alveolar epithelial cells (A549 cells) were analyzed semiquantitatively by RT-PCR. RSV enhanced IRF-1 and iNOS mRNA expression as early as 4 h after RSV infection and this enhancement lasted several hours. No IFN-γ gene expression was observed during the whole course of the infection. Expression of IFN-β, IL-1β, and TNF-α genes was observed slightly at 4 h and became marked 7 h after infection. Addition of neutralizing antibodies to these cytokines to the culture had no effect on the induction of iNOS mRNA. The iNOS transcriptional activity in RSV-infected cells was significantly enhanced by an exogenous cytokine mixture (IL-1β, TNF-α, and IFN-γ). An apparent nitric oxide (NO) production was identified only when cytokines were added together with RSV infection. A significant increase of iNOS gene expression was observed in nasopharyngeal exudate cells obtained from infants during the acute phase of RSV bronchiolitis. These observations suggest that RSV infection of human respiratory epithelial cells induces the iNOS gene both in vitro and in vivo; this induction may occur rather promptly and involves transcriptional activator IRF-1 induced by the RSV infection itself. The iNOS gene, which is initially induced by RSV infection, may be further enhanced in a paracrine fashion by proinflammatory cytokines released by infection-activated inflammatory cells. J. Leukoc. Biol. 66: 99–104; 1999. |
| تدمد: | 1938-3673 0741-5400 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f511b7bbc17dc9d6ff150c07c5f612ea https://doi.org/10.1002/jlb.66.1.99 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f511b7bbc17dc9d6ff150c07c5f612ea |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19383673 07415400 |
|---|