Junín virus infection impairs stress-granule formation in Vero cells treated with arsenite via inhibition of eIF2α phosphorylation
| العنوان: | Junín virus infection impairs stress-granule formation in Vero cells treated with arsenite via inhibition of eIF2α phosphorylation |
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| المؤلفون: | María Gabriela Thomas, Luis Alberto Scolaro, Florencia N. Linero, Graciela Lidia Boccaccio |
| المصدر: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| بيانات النشر: | Microbiology Society, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Thapsigargin, Arsenites, Eukaryotic Initiation Factor-2, Junin Virus, translation, Cytoplasmic Granules, Transfection, Hemorrhagic Fever, American, purl.org/becyt/ford/1 [https], Ciencias Biológicas, chemistry.chemical_compound, SGs, Stress granule, Virology, Cellular stress response, Chlorocebus aethiops, Animals, RNA, Messenger, Phosphorylation, purl.org/becyt/ford/1.6 [https], Vero Cells, eIF2, Junin virus, Arenavirus, biology, Bioquímica y Biología Molecular, biology.organism_classification, Molecular biology, chemistry, Vero cell, eIF2alpha, CIENCIAS NATURALES Y EXACTAS, Plasmids |
| الوصف: | Stress granules (SGs) are ephemeral cytoplasmic aggregates containing stalled translation preinitiation complexes involved in mRNA storage and triage during the cellular stress response. SG formation is triggered by the phosphorylation of the alpha subunit of eIF2 (eIF2α), which provokes a dramatic blockage of protein translation. Our results demonstrate that acute infection of Vero cells with the arenavirus Junín (JUNV), aetiological agent of Argentine haemorrhagic fever, does not induce the formation of SGs. Moreover, JUNV negatively modulates SG formation in infected cells stressed with arsenite, and this inhibition correlates with low levels of eIF2α phosphorylation. Transient expression of JUNV nucleoprotein (N) or the glycoprotein precursor (GPC), but not of the matrix protein (Z), inhibits SG formation in a similar manner, comparable to infectious virus. Expression of N and GPC also impaired eIF2α phosphorylation triggered by arsenite. A moderate inhibition of SG formation was also observed when DTT and thapsigargin were employed as stress inducers. In contrast, no inhibition was observed when infected cells were treated with hippuristanol, a translational inhibitor and inducer of SGs that bypasses the requirement for eIF2α phosphorylation. Finally, we analysed SG formation in persistently JUNV-infected cells, where N and GPC are virtually absent and truncated N products are expressed abundantly. We found that persistently infected cells show a quite normal response to arsenite, with SG formation comparable to that of uninfected cells. This suggests that the presence of GPC and/or N is crucial to control the stress response upon JUNV infection of Vero cells. Fil: Linero, Florencia Natalia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina Fil: Thomas, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina Fil: Boccaccio, Graciela Lidia. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina Fil: Scolaro, Luis Alberto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina |
| وصف الملف: | application/pdf |
| تدمد: | 1465-2099 0022-1317 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f58b866cefdc1e5b18c20e6097bb55d9 https://doi.org/10.1099/vir.0.033407-0 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....f58b866cefdc1e5b18c20e6097bb55d9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14652099 00221317 |
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