Calcium-Induced Membrane Microdomains Trigger Plant Phospholipase D Activity
| العنوان: | Calcium-Induced Membrane Microdomains Trigger Plant Phospholipase D Activity |
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| المؤلفون: | Andreas Kerth, Renate Ulbrich-Hofmann, Konstantin Kuppe, Alfred Blume |
| المصدر: | ChemBioChem. 9:2853-2859 |
| بيانات النشر: | Wiley, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Membrane lipids, Phospholipid, chemistry.chemical_element, Brassica, Biology, Calcium, Biochemistry, Phase Transition, Calcium Chloride, Membrane Lipids, chemistry.chemical_compound, Membrane Microdomains, Phospholipase D, Phospholipase D activity, Molecular Biology, POPC, Calorimetry, Differential Scanning, Vesicle, Organic Chemistry, technology, industry, and agriculture, Phosphatidic acid, Cell biology, chemistry, Molecular Medicine, lipids (amino acids, peptides, and proteins) |
| الوصف: | Plant alpha-type phospholipase D proteins are calcium-dependent, lipolytic enzymes. The morphology of the aggregates of their phospholipid substrate fundamentally defines the interaction between the enzyme and the surface. Here we demonstrate that the Ca(2+)-induced generation of membrane microdomains dramatically activates alpha-type phospholipase D from white cabbage. 500-fold stimulation was observed upon incorporation of 10 mol % 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate (POPA) into 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) vesicles in the presence of Ca(2+) ions. Enhanced association of PLDalpha2 with phospholipid surfaces containing anionic components was indicated by lag phase analysis and film balance measurements. Differential scanning calorimetry showed that the POPA-specific activation correlates with the phase behavior of the POPC/POPA vesicles in the presence of Ca(2+) ions. We conclude from the results that the Ca(2+)-induced formation of POPA microdomains is the crucial parameter that facilitates the binding of PLD to the phospholipid surface and suggest that this effect serves as a cellular switch for controlling PLD activity. |
| تدمد: | 1439-7633 1439-4227 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5918109e7efc24b9fae78caf5faa301 https://doi.org/10.1002/cbic.200800431 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....f5918109e7efc24b9fae78caf5faa301 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14397633 14394227 |
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